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Fast 3-D dark-field reflection-mode photoacoustic microscopy in vivo with a 30-MHz ultrasound linear array

Song, Liang and Maslov, Konstantin and Bitton, Rachel and Shung, K. Kirk and Wang, Lihong V. (2008) Fast 3-D dark-field reflection-mode photoacoustic microscopy in vivo with a 30-MHz ultrasound linear array. Journal of Biomedical Optics, 13 (5). Art. No. 054028. ISSN 1083-3668. PMCID PMC2644744. doi:10.1117/1.2976141.

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We present an in vivo dark-field reflection-mode photoacoustic microscopy system that performs cross-sectional (B-scan) imaging at 50Hz with real-time beamforming and 3-D imaging consisting of 166 B-scan frames at 1Hz with postbeamforming. To our knowledge, this speed is currently the fastest in photoacoustic imaging. A custom-designed light delivery system is integrated with a 30-MHz ultrasound linear array to realize dark-field reflection-mode imaging. Linear mechanical scanning of the array produces 3-D images. The system has axial, lateral, and elevational resolutions of 25, 70, and 200μm, respectively, and can image 3mm deep in scattering biological tissues. Volumetric images of subcutaneous vasculature in rats are demonstrated in vivo. Fast 3-D photoacoustic microscopy is anticipated to facilitate applications of photoacoustic imaging in biomedical studies that involve dynamics and clinical procedures that demand immediate diagnosis.

Item Type:Article
Related URLs:
URLURL TypeDescription CentralArticle
Maslov, Konstantin0000-0003-3408-8840
Wang, Lihong V.0000-0001-9783-4383
Additional Information:© 2008 SPIE. Paper 08069R received Feb. 28, 2008; revised manuscript received May 22, 2008; accepted for publication May 23, 2008; published online Sep. 11, 2008. This work was sponsored, in part, by Grants No. R01 EB000712 and No. R01 NS46214 from the National Institutes of Health.
Funding AgencyGrant Number
NIHR01 EB000712
NIHR01 NS46214
Subject Keywords:biomedical imaging; realtime photoacoustic imaging; three-dimensional photoacoustic microscopy; high-frequency ultrasound array; clinical diagnosis; subcutaneous vasculature; small animal imaging
Issue or Number:5
PubMed Central ID:PMC2644744
Record Number:CaltechAUTHORS:20160923-110234128
Persistent URL:
Official Citation:Song L, Maslov K, Bitton R, Shung K, Wang LV; Fast 3-d dark-field reflection-mode photoacoustic microscopy in vivo with a 30-mhz ultrasound linear array. J. Biomed. Opt. 0001;13(5):054028-054028-5
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:70558
Deposited By: Tony Diaz
Deposited On:28 Sep 2016 23:42
Last Modified:11 Nov 2021 04:31

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