Recognition of Ribosomal RNA sites in DNA, II. The Hela Cell System

Abstract

As reported in a preceding paper,[1] the analysis of the sedimentation behavior and base composition of the RNA recovered from hybrids between E. coli 16S and 238 RNA and homologous DNA, after RNase digestion of non-base-paired segments, has given results which suggest a regular and complete hydrogen bonding of the hybridized RNA with specific sites in DNA. A similar analysis has now been applied to the investigation of ribosomal RNA sites in HeLa and other human DNA's and has made it possible to resolve the ambiguities in specificity pattern and base composition presented by the raw hybridization data, and to identify among the RNA-DNA complexes a fraction which appears to involve specific sites in DNA.