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Diffraction tomography with Fourier ptychography

Horstmeyer, Roarke and Chung, Jaebum and Ou, Xiaoze and Zheng, Guoan and Yang, Changhuei (2016) Diffraction tomography with Fourier ptychography. Optica, 3 (8). pp. 827-835. ISSN 2334-2536. PMCID PMC5521281.

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This paper presents a technique to image the complex index of refraction of a sample across three dimensions. The only required hardware is a standard microscope and an array of LEDs. The method, termed Fourier ptychographic tomography (FPT), first captures a sequence of intensity-only images of a sample under angularly varying illumination. Then, using principles from ptychography and diffraction tomography, it computationally solves for the sample structure in three dimensions. The experimental microscope demonstrates a lateral spatial resolution of 0.39 μm and an axial resolution of 3.7 μm at the Nyquist–Shannon sampling limit (0.54 and 5.0 μm at the Sparrow limit, respectively) across a total imaging depth of 110 μm. Unlike competing methods, this technique quantitatively measures the volumetric refractive index of primarily transparent and contiguous sample features without the need for interferometry or any moving parts. Wide field-of-view reconstructions of thick biological specimens suggest potential applications in pathology and developmental biology.

Item Type:Article
Related URLs:
URLURL TypeDescription Information CentralArticle
Horstmeyer, Roarke0000-0002-2480-9141
Chung, Jaebum0000-0003-3932-8428
Yang, Changhuei0000-0001-8791-0354
Additional Information:© 2016 Optical Society of America. Received 2 May 2016; revised 3 July 2016; accepted 3 July 2016 (Doc. ID 264094); published 27 July 2016. The authors would like to thank J. Brake, B. Judkewitz, and I. Papadopoulos for the helpful discussions and feedback. Funding: National Institutes of Health (NIH) (1R01AI096226-01); The Caltech Innovation Initiative (CI2) Program (13520135).
Funding AgencyGrant Number
Caltech Innovation Initiative (CI2)13520135
Issue or Number:8
Classification Code:OCIS codes: (180.6900) Three-dimensional microscopy; (110.6955) Tomographic imaging.
PubMed Central ID:PMC5521281
Record Number:CaltechAUTHORS:20160930-124858247
Persistent URL:
Official Citation:Roarke Horstmeyer, Jaebum Chung, Xiaoze Ou, Guoan Zheng, and Changhuei Yang, "Diffraction tomography with Fourier ptychography," Optica 3, 827-835 (2016)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:70694
Deposited By: George Porter
Deposited On:30 Sep 2016 20:26
Last Modified:09 Mar 2020 13:19

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