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Atomic Force Microscopy Imaging and Electrical Recording of Lipid Bilayers Supported over Microfabricated Silicon Chip Nanopores: Lab-on-a-Chip System for Lipid Membranes and Ion Channels

Quist, Arjan P. and Chand, Ami and Ramachandran, Srinivasan and Daraio, Chiara and Jin, Sungho and Lal, Ratnesh (2007) Atomic Force Microscopy Imaging and Electrical Recording of Lipid Bilayers Supported over Microfabricated Silicon Chip Nanopores: Lab-on-a-Chip System for Lipid Membranes and Ion Channels. Langmuir, 23 (3). pp. 1375-1380. ISSN 0743-7463. https://resolver.caltech.edu/CaltechAUTHORS:20161003-151554509

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Abstract

We describe a silicon chip-based supported bilayer system to detect the presence of ion channels and their electrical conductance in lipid bilayers. Nanopores were produced in microfabricated silicon membranes by electron beam lithography as well as by using a finely focused ion beam. Thermal oxide was used to shrink pore sizes, if necessary, and to create an insulating surface. The chips with well-defined pores were easily mounted on a double-chamber plastic cell recording system, allowing for controlling the buffer conditions both above and below the window. The double-chamber system allowed using an atomic force microscopy (AFM) tip as one electrode and inserting a platinum wire as the second electrode under the membrane window, to measure electrical current across lipid bilayers that are suspended over the pores. Atomic force imaging, stiffness measurement, and electrical capacitance measurement show the feasibility of supporting lipid bilayers over defined nanopores:  a key requirement to use any such technique for structure−function study of ion channels. Online addition of gramicidin, an ion-channel-forming peptide, resulted in electrical current flow across the bilayer, and the I−V curve that was measured using the conducting AFM tip indicates the presence of many conducting gramicidin ion channels.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1021/la062187zDOIArticle
http://pubs.acs.org/doi/abs/10.1021/la062187zPublisherArticle
ORCID:
AuthorORCID
Daraio, Chiara0000-0001-5296-4440
Additional Information:© 2007 American Chemical Society. Received July 25, 2006. In Final Form: October 15, 2006. Publication Date (Web): December 8, 2006. We thank Raymond Lee of FIB International for performing the focus ion beam milling procedure. The work was supported by grants from the NIH, California State Department of Health, and Philip Morris External Grant program.
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Funding AgencyGrant Number
NIHUNSPECIFIED
California State Department of HealthUNSPECIFIED
Philip Morris External Research ProgramUNSPECIFIED
Issue or Number:3
Record Number:CaltechAUTHORS:20161003-151554509
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20161003-151554509
Official Citation:Atomic Force Microscopy Imaging and Electrical Recording of Lipid Bilayers Supported over Microfabricated Silicon Chip Nanopores:  Lab-on-a-Chip System for Lipid Membranes and Ion Channels Arjan P. Quist,†,‡, Ami Chand,§, Srinivasan Ramachandran,†, Chiara Daraio,‖, Sungho Jin,‖ and, and Ratnesh Lal*,† Langmuir 2007 23 (3), 1375-1380 DOI: 10.1021/la062187z
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:70770
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:03 Oct 2016 22:30
Last Modified:03 Oct 2019 16:01

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