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Molecular contrast optical coherence tomography: a pump-probe scheme using indocyanine green as a contrast agent

Yaqoob, Zahid and McDowell, Emily J. and Wu, Jigang and Heng, Xin and Fingler, Jeff and Yang, Changhuei (2006) Molecular contrast optical coherence tomography: a pump-probe scheme using indocyanine green as a contrast agent. Journal of Biomedical Optics, 11 (5). Art. No. 054017. ISSN 1083-3668. https://resolver.caltech.edu/CaltechAUTHORS:20161005-135059869

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Abstract

The use of indocyanine green (ICG), a U.S. Food and Drug Administration approved dye, in a pump-probe scheme for molecular contrast optical coherence tomography (MCOCT) is proposed and demonstrated for the first time. In the proposed pump-probe scheme, an optical coherence tomography (OCT) scan of the sample containing ICG is first acquired. High fluence illumination (∼190kJ/cm^2) is then used to permanently photobleach the ICG molecules—resulting in a permanent alteration of the overall absorption of the ICG. A second OCT scan is next acquired. The difference of the two OCT scans is used to determine the depth resolved distribution of ICG within a sample. To characterize the extent of photobleaching in different ICG solutions, we determine the cumulative probability of photobleaching, ϕ_(B,cum), defined as the ratio of the total photobleached ICG molecules to the total photons absorbed by the ground state molecules. An empirical study of ICG photobleaching dynamics shows that ϕ_(B,cum) decreases with fluence as well as with increasing dye concentration. The quantity ϕ_(B,cum) is useful for estimating the extent of photobleaching in an ICG sample (MCOCT contrast) for a given fluence of the pump illumination. The paper also demonstrates ICG-based MCOCT imaging in tissue phantoms as well as within stage 54 Xenopus laevis.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1117/1.2360525DOIArticle
http://biomedicaloptics.spiedigitallibrary.org/article.aspx?articleid=1102301PublisherArticle
ORCID:
AuthorORCID
Yang, Changhuei0000-0001-8791-0354
Additional Information:© 2006 SPIE. Received October 17, 2005; Revised May 13, 2006; Accepted June 20, 2006; Published October 26, 2006; Online October 26, 2006. This research was supported by National Institute of Health Grant No. 1RZ1EB004602-1.
Funders:
Funding AgencyGrant Number
NIH1RZ1EB004602-1
Subject Keywords:optical coherence tomography; molecular contrast; indocyanine green; medical and biological imaging; pump-probe
Issue or Number:5
Record Number:CaltechAUTHORS:20161005-135059869
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20161005-135059869
Official Citation:Molecular contrast optical coherence tomography: a pump-probe scheme using indocyanine green as a contrast agent Zahid Yaqoob; Emily McDowell; Jigang Wu; Xin Heng; Jeff Fingler; Changhuei Yang J. Biomed. Opt. 11 (5), 054017 (October 26, 2006); doi: 10.1117/1.2360525
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:70882
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:05 Oct 2016 21:17
Last Modified:09 Mar 2020 13:19

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