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Dissecting the transcriptional regulatory network for early T-cell commitment

Rothenberg, E. and Kueh, H. Y. and Hosokawa, H. and Ng, K. K. H. and Mehta, A. and Pease, S. S. and Romero-Wolf, M. and Ungerbäck, J. and Wang, X. and Yui, M. A. and Zeng, W. B. and Elowitz, M. B. and Mortazavi, A. (2016) Dissecting the transcriptional regulatory network for early T-cell commitment. European Journal of Immunology, 46 (S1). p. 797. ISSN 0014-2980. doi:10.1002/eji.201670200.

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Multipotent precursors proliferating in the thymus become committed to a T-cell fate when their access to alternative cell fates is intrinsically and unconditionally closed. This process involves very dynamic changes in transcription factor expression and activity: PU.1 and other factors expressed in multipotent progenitors are downregulated and are usually permanently silenced, while T-cell transcription factors, especially Bcl11b, are sharply activated. The abrupt activation of Bcl11b from a silent state is not only a landmark in T-cell differentiation, marking commitment, but also a functional contributor to commitment. Key target genes affected by Bcl11b function at this particular stage will be described: many of them appear to be under the control of Bcl11b as a repressor. Recent proteomic characterization of Bcl11b-containing protein complexes indicates the corepressor and coactivator partners that it uses to mediate different types of regulatory activity at different genomic sites. Since Bcl11b is such a powerful factor, a central question is how the onset of expression of Bcl11b itself is controlled. The talk will describe the combination of trans-acting factors that are needed to open the Bcl11b locus and activate it, and show that these factors each play slightly different temporal and functional roles in the activation process. Interestingly, evidence will also be shown for an epigenetic component of Bcl11b regulation that further slows its activation but helps to make it irreversible.

Item Type:Article
Related URLs:
URLURL TypeDescription
Rothenberg, E.0000-0002-3901-347X
Kueh, H. Y.0000-0001-6272-6673
Hosokawa, H.0000-0002-9592-2889
Romero-Wolf, M.0000-0002-8024-7198
Yui, M. A.0000-0002-3136-2181
Elowitz, M. B.0000-0002-1221-0967
Mortazavi, A.0000-0002-4259-6362
Additional Information:© 2016 The Authors. European Journal of Immunology © Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim. First published: 19 August 2016.
Issue or Number:S1
Record Number:CaltechAUTHORS:20161021-124515838
Persistent URL:
Official Citation:(2016), Abstracts of ICI 2016 International Congress of Immunology, 21–26 August 2016, Melbourne, Australia. Eur. J. Immunol., 46: 1–1238. doi:10.1002/eji.201670200
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:71347
Deposited By: Tony Diaz
Deposited On:21 Oct 2016 20:24
Last Modified:11 Nov 2021 04:43

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