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Hippocampal to basal forebrain transport of Mn^(2+) is impaired by deletion of KLC1, a subunit of the conventional kinesin microtubule-based motor

Medina, Christopher S. and Biris, Octavian and Falzone, Tomas L. and Zhang, Xiaowei and Zimmerman, Amber J. and Bearer, Elaine L. (2017) Hippocampal to basal forebrain transport of Mn^(2+) is impaired by deletion of KLC1, a subunit of the conventional kinesin microtubule-based motor. NeuroImage, 145 (Pt. A). pp. 44-57. ISSN 1053-8119. http://resolver.caltech.edu/CaltechAUTHORS:20161024-101809831

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Abstract

Microtubule-based motors carry cargo back and forth between the synaptic region and the cell body. Defects in axonal transport result in peripheral neuropathies, some of which are caused by mutations in KIF5A, a gene encoding one of the heavy chain isoforms of conventional kinesin-1. Some mutations in KIF5A also cause severe central nervous system defects in humans. While transport dynamics in the peripheral nervous system have been well characterized experimentally, transport in the central nervous system is less experimentally accessible and until now not well described. Here we apply manganese-enhanced magnetic resonance (MEMRI) to study transport dynamics within the central nervous system, focusing on the hippocampal-forebrain circuit, and comparing kinesin-1 light chain 1 knock-out (KLC-KO) mice with age-matched wild-type littermates. We injected Mn^(2+) into CA3 of the posterior hippocampus and imaged axonal transport in vivo by capturing whole-brain 3D magnetic resonance images (MRI) in living mice at discrete time-points after injection. Precise placement of the injection site was monitored in both MR images and in histologic sections. Mn^(2+)-induced intensity progressed along fiber tracts (fimbria and fornix) in both genotypes to the medial septal nuclei (MSN), correlating in location with the traditional histologic tract tracer, rhodamine dextran. Pairwise statistical parametric mapping (SPM) comparing intensities at successive time-points within genotype revealed Mn^(2+)-enhanced MR signal as it proceeded from the injection site into the forebrain, the expected projection from CA3. By region of interest (ROI) analysis of the MSN, wide variation between individuals in each genotype was found. Despite this statistically significant intensity increases in the MSN at 6 hr post-injection was found in both genotypes, albeit less so in the KLC-KO. While the average accumulation at 6 hr was less in the KLC-KO, this difference did not reach significance. Projections of SPM T-maps for each genotype onto the same grayscale image revealed differences in the anatomical location of significant voxels. Although KLC-KO mice had smaller brains than wild-type, the gross anatomy was normal with no apparent loss of septal cholinergic neurons. Hence anatomy alone does not explain the differences in SPM maps. We conclude that kinesin-1 defects may have only a minor effect on the rate and distribution of transported Mn^(2+) within the living brain. This impairment is less than expected for this abundant microtubule-based motor, yet such defects could still be functionally significant, resulting in cognitive/emotional dysfunction due to decreased replenishments of synaptic vesicles or mitochondria during synaptic activity. This study demonstrates the power of MEMRI to observe and measure vesicular transport dynamics in the central nervous system that may result from or lead to brain pathology.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/j.neuroimage.2016.09.035DOIArticle
http://www.sciencedirect.com/science/article/pii/S1053811916304979PublisherArticle
Additional Information:© 2016 Elsevier B.V. Received 9 March 2016; accepted 15 September 2016; available online 14 October 2016. The authors gratefully acknowledge Ivo Dinov and Joshi Anand in the Laboratory of NeuroImaging directed by Art Toga for assistance with the LONI Pipeline processing environment; Kevin Reagan, Paulette Ferland, Abdul Faheem Mohed and Kathleen Kilpatrick for technical assistance; and Brett Manifold-Wheeler for helpful discussions. We thank Russell E. Jacobs (California Institute of Technology and the Beckman Institute Small Animal Imaging Facility) for assistance with data collection, analysis and useful comments, and to Vince Calhoun, at UNM and Mind Research Network, for help with SPM analysis. We thank Louie Kerr and the Central Microscopy Facility at the Marine Biological Laboratory for access to their equipment, and to the MBL library where most of this MS was written. We are particularly grateful to Li Luo, our UNM CTSC statistician, for help with statistical analysis and its interpretation. This work is supported by NIH R01 MH096093, RO1 NS062184, P5O GM08273, UNM CTSC grant UL1 TR000041 and the Harvey Family Endowment.
Funders:
Funding AgencyGrant Number
NIHR01 MH096093
NIHRO1 NS062184
NIHP5O GM08273
NIHUL1 TR000041
Harvey Family EndowmentUNSPECIFIED
Subject Keywords:Kinesin-1; Kinesin light chain-1; Axonal transport; Manganese-enhanced magnetic resonance imaging (MEMRI); Hippocampal-forebrain circuit; Statistical parametric mapping
Record Number:CaltechAUTHORS:20161024-101809831
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20161024-101809831
Official Citation:Christopher S. Medina, Octavian Biris, Tomas L. Falzone, Xiaowei Zhang, Amber J. Zimmerman, Elaine L. Bearer, Hippocampal to basal forebrain transport of Mn2+ is impaired by deletion of KLC1, a subunit of the conventional kinesin microtubule-based motor, NeuroImage, Volume 145, Part A, 15 January 2017, Pages 44-57, ISSN 1053-8119, http://dx.doi.org/10.1016/j.neuroimage.2016.09.035. (//www.sciencedirect.com/science/article/pii/S1053811916304979)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:71375
Collection:CaltechAUTHORS
Deposited By: Melissa Ray
Deposited On:24 Oct 2016 18:24
Last Modified:01 Feb 2017 22:50

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