A Caltech Library Service

Engineering the Dynamic Properties of Protein Networks through Sequence Variation

Dooling, Lawrence J. and Tirrell, David A. (2016) Engineering the Dynamic Properties of Protein Networks through Sequence Variation. ACS Central Science, 2 (11). pp. 812-819. ISSN 2374-7943. PMCID PMC5126713. doi:10.1021/acscentsci.6b00205.

[img] PDF (ACS AuthorChoice) - Published Version
See Usage Policy.

[img] PDF (Additional experimental details of cloning, protein characterization, and hydrogel swelling. Supporting Figures S1–S9. Supporting Tables S1–S5) - Supplemental Material
See Usage Policy.


Use this Persistent URL to link to this item:


The dynamic behavior of macromolecular networks dominates the mechanical properties of soft materials and influences biological processes at multiple length scales. In hydrogels prepared from self-assembling artificial proteins, stress relaxation and energy dissipation arise from the transient character of physical network junctions. Here we show that subtle changes in sequence can be used to program the relaxation behavior of end-linked networks of engineered coiled-coil proteins. Single-site substitutions in the coiled-coil domains caused shifts in relaxation time over 5 orders of magnitude as demonstrated by dynamic oscillatory shear rheometry and stress relaxation measurements. Networks with multiple relaxation time scales were also engineered. This work demonstrates how time-dependent mechanical responses of macromolecular materials can be encoded in genetic information.

Item Type:Article
Related URLs:
URLURL TypeDescription Information CentralArticle
Dooling, Lawrence J.0000-0002-1688-2066
Tirrell, David A.0000-0003-3175-4596
Additional Information:© 2016 American Chemical Society. ACS AuthorChoice - This is an open access article published under an ACS AuthorChoice License, which permits copying and redistribution of the article or any adaptations for non-commercial purposes. Received: July 21, 2016. Publication Date (Web): October 18, 2016. This work was supported by Grant Number DMR-1506483 from the Biomaterials Program of the U.S. National Science Foundation. We thank Professor Julia Kornfield for extensive access to the ARES-RFS rheometer and Dr. Mona Shahgholi of the Mass Spectrometry Facility of the Division of Chemistry and Chemical Engineering at Caltech for assistance in measuring protein molar masses. The authors declare no competing financial interest.
Funding AgencyGrant Number
Issue or Number:11
PubMed Central ID:PMC5126713
Record Number:CaltechAUTHORS:20161024-112450940
Persistent URL:
Official Citation:Engineering the Dynamic Properties of Protein Networks through Sequence Variation Lawrence J. Dooling and David A. Tirrell ACS Central Science 2016 2 (11), 812-819 DOI: 10.1021/acscentsci.6b00205
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:71384
Deposited By: George Porter
Deposited On:24 Oct 2016 19:17
Last Modified:14 Apr 2022 16:53

Repository Staff Only: item control page