A Caltech Library Service

Characterization of the cytotoxicity and imaging properties of second-harmonic nanoparticles

Hsieh, Chia-Lung and Grange, Rachel and Pu, Ye and Psaltis, Demetri (2010) Characterization of the cytotoxicity and imaging properties of second-harmonic nanoparticles. In: Biosensing III. Proceedings of SPIE. No.7759. Society of Photo-Optical Instrumentation Engineers (SPIE) , Bellingham, WA, Art. No. 77590T. ISBN 978-0-8194-8255-6.

[img] PDF - Published Version
See Usage Policy.


Use this Persistent URL to link to this item:


We develop second-harmonic nanoparticles as the contrast agents for cell imaging. Second-harmonic nanoparticles show promise as cell imaging probes due to their non-bleaching, non-blinking, and coherent signal. Nanoparticles of noncentrosymmetric crystal structures have high second-harmonic generation (SHG) efficiency and provide high contrast in a generally non-structured cell environment. Here, we use barium titanate (BaTiO_3) nanoparticles with tetragonal crystal structure as imaging probes. Cytotoxicity tests performed on BaTiO_3 nanoparticles with mammalian cells did not result in toxic effects. Specifically, we observed no change in the cell metabolism after 24 hours incubation of the cells with high concentration of BaTiO_3 nanoparticles. We demonstrate two methods of cell labeling with BaTiO_3 nanoparticles for imaging. One is non-specific labeling via endocytosis of the cells, which results in a great number of the nanoparticles randomly distributed inside the cells. The other is specific labeling via surface functionalization of the nanoparticles with antibodies, which enables us to label specific cell membrane proteins with the nanoparticles. SHG imaging is compatible to two-photon microscopy and the SHG signal from nanoparticles can be easily detected with a standard two-photon confocal microscope. Our work provides the opportunity for long-term, three-dimensional cell tracking with secondharmonic nanoparticles.

Item Type:Book Section
Related URLs:
URLURL TypeDescription
Additional Information:© 2010 SPIE The International Society for Optical Engineering. The authors thank Paul Bowen for providing the nanoparticles, Marc Chambon and Nathalie Ballanfat for the help of HeLa cell preparation and cytotoxicity test. We also like to thank Eric Bernasconi and Dominique Velin at the Gastroenterology division in CHUV for the myeloid cells preparation. This project is supported by National Center of Competence in Research (NCCR), Quantum Photonics.
Funding AgencyGrant Number
National Center of Competence in Research (NCCR) Quantum PhotonicsUNSPECIFIED
Subject Keywords:Second harmonic generation, barium titanate, nanoparticle, microscopy, biological cells, cytotoxicity, endocytosis, surface functionalization
Series Name:Proceedings of SPIE
Issue or Number:7759
Record Number:CaltechAUTHORS:20161028-153246667
Persistent URL:
Official Citation:Chia-Lung Hsieh ; Rachel Grange ; Ye Pu ; Demetri Psaltis; Characterization of the cytotoxicity and imaging properties of second-harmonic nanoparticles. Proc. SPIE 7759, Biosensing III, 77590T (August 24, 2010); doi:10.1117/12.860595.
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:71597
Deposited By: Ruth Sustaita
Deposited On:31 Oct 2016 15:00
Last Modified:03 Oct 2019 16:09

Repository Staff Only: item control page