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Quantitative assessments of glycolysis from single cells

Shin, Young Shik and Kim, Jungwoo and Johnson, Dazy and Dooraghi, Alex A. and Mai, Wilson X. and Ta, Lisa and Chatziioannou, Arion F. and Phelps, Michael E. and Nathanson, David A. and Heath, James R. (2015) Quantitative assessments of glycolysis from single cells. Technology, 3 (4). pp. 172-178. ISSN 2339-5478. PMCID PMC4728151. https://resolver.caltech.edu/CaltechAUTHORS:20161031-083900031

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Abstract

The most common positron emission tomography (PET) radio-labeled probe for molecular diagnostics in patient care and research is the glucose analog, 2-deoxy-2-[F-18]fluoro-D-glucose (^(18)F-FDG). We report on an integrated microfluidics-chip/beta particle imaging system for in vitro ^(18)F-FDG radioassays of glycolysis with single cell resolution. We investigated the kinetic responses of single glioblastoma cancer cells to targeted inhibitors of receptor tyrosine kinase signaling. Further, we find a weak positive correlation between cell size and rate of glycolysis.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1142/S2339547815200058 DOIArticle
http://www.worldscientific.com/doi/10.1142/S2339547815200058PublisherArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4728151/PubMed CentralArticle
ORCID:
AuthorORCID
Kim, Jungwoo0000-0002-5215-2044
Johnson, Dazy0000-0001-6311-8629
Heath, James R.0000-0001-5356-4385
Additional Information:© 2015 World Scientific Publishing Co Pte Ltd. Received: 14 March 2015; Accepted: 5 May 2015; Published: 25 June 2015. This work was supported by the National Cancer Institute grant 5U54 CA151819 (JRH PI), the Ben and Catherine Ivy Foundation, the Jean Perkins Foundation, the NCI In Vivo Cellular and Molecular Imaging Center (ICMIC) and the Phelps Family Foundation. A.D. was supported in part by the UCLA Scholars in Oncologic Molecular Imaging program, NIH grant R25T CA098010. Y.S.S. acknowledges the support from the Korean-American Scientists and Engineers Association (KSEA). Author Contributions: Y.S.S. and J.K. developed microfluidic device, designed and performed tests. D.J., W.X.M., and L.T. prepared biological samples for tests. A.A.D. and A.F.C. developed the Betabox camera and software. D.A.N. and M.E.P. provided detailed guidelines and discussion for the experimental design and interpretation of the results. Y.S.S., J.K., and J.R.H. wrote the manuscript. J.R.H. and Y.S.S. directed the research. Competing Interests Statement: M.E.P., A.F.C., and J.R.H. are founders and stockholders in Sofie Bio-sciences, Inc., which is seeking to commercialize certain aspects of the Betabox technology.
Funders:
Funding AgencyGrant Number
NIH5U54 CA151819
Ben and Catherine Ivy FoundationUNSPECIFIED
Jean Perkins FoundationUNSPECIFIED
National Cancer InstituteUNSPECIFIED
Phelps Family FoundationUNSPECIFIED
UCLAUNSPECIFIED
NIHR25T CA098010
Korean-American Scientists and Engineers AssociationUNSPECIFIED
Issue or Number:4
PubMed Central ID:PMC4728151
Record Number:CaltechAUTHORS:20161031-083900031
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20161031-083900031
Official Citation:Quantitative assessments of glycolysis from single cells Young Shik Shin, Jungwoo Kim, Dazy Johnson, Alex A. Dooraghi, Wilson X. Mai, Lisa Ta, Arion F. Chatziioannou, Michael E. Phelps, David A. Nathanson, and James R. Heath TECHNOLOGY 2015 03:04, 172-178
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:71603
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:31 Oct 2016 16:02
Last Modified:09 Mar 2020 13:18

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