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Solving the “World-to-Chip” Interface Problem with a Microfluidic Matrix

Liu, Jian and Hansen, Carl L. and Quake, Stephen R. (2003) Solving the “World-to-Chip” Interface Problem with a Microfluidic Matrix. Analytical Chemistry, 75 (18). pp. 4718-4723. ISSN 0003-2700. doi:10.1021/ac0346407.

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We report an effective solution to the macroscopic/microfluidic interface issue and demonstrate how microfluidics can achieve impressive economies of scale in reducing the complexity of pipetting operations. Using an N × N microfluidic matrix with N = 20, we performed N^2 = 400 distinct PCR reactions with only 2N + 1 = 41 pipetting steps, compared with the 3N^2 = 1200 steps required with conventional fluid handling. Each vertex of the matrix has a 3-nL reactor, and a single 2-μL aliquot of polymerase is amortized over all 400 independent reactions, thus dramatically reducing sample overhead and minimizing reagent consumption. Beyond PCR, the matrix chip provides a general method to perform chemical and biological experiments with precious reagents in a highly automated fashion.

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Liu, Jian0000-0001-8552-1400
Additional Information:© 2003 American Chemical Society. Received for review June 11, 2003. Accepted August 1, 2003. We thank Marc Unger and Emily Lin for helpful discussions. This work was supported in part by the DARPA Bioflips and by the NSF XYZ on a Chip programs.
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Defense Advanced Research Projects Agency (DARPA)UNSPECIFIED
Issue or Number:18
Record Number:CaltechAUTHORS:20161102-145926798
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Official Citation:Solving the “World-to-Chip” Interface Problem with a Microfluidic Matrix Jian Liu, Carl Hansen, and Stephen R. Quake Analytical Chemistry 2003 75 (18), 4718-4723 DOI: 10.1021/ac0346407
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:71686
Deposited By: Ruth Sustaita
Deposited On:02 Nov 2016 23:46
Last Modified:11 Nov 2021 04:49

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