CaltechAUTHORS
  A Caltech Library Service

Functional opsin retrogene in nocturnal moth

Xu, Pengjun and Feuda, Roberto and Lu, Bin and Xiao, Haijun and Graham, Robert I. and Wu, Kongming (2016) Functional opsin retrogene in nocturnal moth. Mobile DNA, 7 . Art. No. 18. ISSN 1759-8753. PMCID PMC5070202. http://resolver.caltech.edu/CaltechAUTHORS:20161109-132235267

[img] PDF - Published Version
Creative Commons Attribution.

765Kb
[img] MS Word - Supplemental Material
Creative Commons Attribution.

2663Kb
[img] Other (Additional file 2: Alignment of sequences used in this study. (PHY)) - Supplemental Material
Creative Commons Attribution.

100Kb

Use this Persistent URL to link to this item: http://resolver.caltech.edu/CaltechAUTHORS:20161109-132235267

Abstract

Background: Retrotransposed genes are different to other types of genes as they originate from a processed mRNA and are then inserted back into the genome. For a long time, the contribution of this mechanism to the origin of new genes, and hence to the evolutionary process, has been questioned as retrogenes usually lose their regulatory sequences upon insertion and generally decay into pseudogenes. In recent years, there is growing evidence, notably in mammals, that retrotransposition is an important process driving the origin of new genes, but the evidence in insects remains largely restricted to a few model species. Findings: By sequencing the messenger RNA of three developmental stages (first and fifth instar larvae and adults) of the pest Helicoverpa armigera, we identified a second, intronless, long-wavelength sensitive opsin (that we called LWS2). We then amplified the partial CDS of LWS2 retrogenes from another six noctuid moths, and investigate the phylogenetic distribution of LWS2 in 15 complete Lepidoptera and 1 Trichoptera genomes. Our results suggests that LWS2 evolved within the noctuid. Furthermore, we found that all the LWS2 opsins have an intact ORF, and have an ω-value (ω = 0.08202) relatively higher compared to their paralog LWS1 (ω = 0.02536), suggesting that LWS2 opsins were under relaxed purifying selection. Finally, the LWS2 shows temporal compartmentalization of expression. LWS2 in H. armigera in adult is expressed at a significantly lower level compared to all other opsins in adults; while in the in 1st instar stage larvae, it is expressed at a significantly higher level compared to other opsins. Conclusions: Together the results of our evolutionary sequence analyses and gene expression data suggest that LWS2 is a functional gene, however, the relatively low level of expression in adults suggests that LWS2 is most likely not involved in mediating the visual process.


Item Type:Article
Related URLs:
URLURL TypeDescription
http:dx.doi.org/10.1186/s13100-016-0074-8DOIArticle
http://mobilednajournal.biomedcentral.com/articles/10.1186/s13100-016-0074-8PublisherArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5070202/PubMed CentralArticle
Additional Information:© 2016 The Author(s). This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. Received: 21 June 2016 Accepted: 21 September 2016. Published online: 19 October 2016. We thanks Dr. Tiantao Zhang, from Institute of Plant Protection, Chinese Academy of Agricultural Sciences, for providing the reference sequence of LWS1 and LWS2 in O. nubilalis. This research was supported by Science Fund for Creative Research Groups of the National Science Foundation of China (No. 31321004), the National Natural Science Foundation of China (Grant No. 31401752). Authors’ contributions: KW, PX, RF, BL and HX conceived the study. PX, BL and HX performed the experiments. KW, PX, RF, BL, HX and RIG wrote the manuscript. All of the authors critically reviewed and approved the final manuscript. The authors declare that they have no competing interests.
Funders:
Funding AgencyGrant Number
National Science Foundation of China31321004
National Natural Science Foundation of China31401752
PubMed Central ID:PMC5070202
Record Number:CaltechAUTHORS:20161109-132235267
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20161109-132235267
Official Citation:Xu P, Feuda R, Lu B, Xiao H, Graham RI, Wu K. Functional opsin retrogene in nocturnal moth. Mobile DNA. 2016;7:18. doi:10.1186/s13100-016-0074-8.
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:71888
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:09 Nov 2016 22:09
Last Modified:09 Nov 2016 22:09

Repository Staff Only: item control page