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The Placental Interleukin-6 Signaling Controls Fetal Brain Development and Behavior

Wu, Wei-Li and Hsiao, Elaine Y. and Yan, Zihao and Mazmanian, Sarkis K. and Patterson, Paul H. (2017) The Placental Interleukin-6 Signaling Controls Fetal Brain Development and Behavior. Brain, Behavior, and Immunity, 62 . pp. 11-23. ISSN 0889-1591. PMCID PMC5373986. https://resolver.caltech.edu/CaltechAUTHORS:20161114-135126065

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Abstract

Epidemiological studies show that maternal immune activation (MIA) during pregnancy is a risk factor for autism. However, mechanisms for how MIA affects brain development and behaviors in offspring remain poorly described. To determine whether placental interleukin-6 (IL-6) signaling is required for mediating MIA on the offspring, we generated mice with restricted deletion of the receptor for IL-6 (IL-6R_) in placental trophoblasts (Cyp19-Cre^(+);Il6ra^(fl/fl)), and tested offspring of Cyp19-Cre^(+);Il6ra^(fl/fl) mothers for immunological, pathological and behavioral abnormalities following induction of MIA. We reveal that MIA results in acute inflammatory responses in the fetal brain. Lack of IL-6 signaling in trophoblasts effectively blocks MIA-induced inflammatory responses in the placenta and the fetal brain. Furthermore, behavioral abnormalities and cerebellar neuropathologies observed in MIA control offspring are prevented in Cyp19-Cre^(+);Il6ra^(fl/fl) offspring. Our results demonstrate that IL-6 activation in placenta is required for relaying inflammatory signals to the fetal brain and impacting behaviors and neuropathologies relevant to neurodevelopmental disease.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/j.bbi.2016.11.007DOIArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5373986/PubMed CentralArticle
ORCID:
AuthorORCID
Wu, Wei-Li0000-0003-2610-1881
Hsiao, Elaine Y.0000-0002-1633-588X
Mazmanian, Sarkis K.0000-0003-2713-1513
Additional Information:© 2016 Elsevier. Received 27 July 2016; revised 20 October 2016; accepted 8 November 2016; available online 9 November 2016. We acknowledge H. Chu, A. Khoshnan, B.D. Needham, T.R. Sampson, C.E. Schretter, and G. Sharon for critically reviewing the manuscript; Professor Gustavo Leone for providing the Cyp19-Cre mouse line; Professor D.J. Anderson for providing ROSA::LSLlacZ mice; Professor A.J. Varshavsky for providing Ate1^(-/-) mouse; Professor J.M. Allman for use of the LCM equipment; Professor B.J. Wold for use of a cryostat; Dr. B. Williams for training and use of LCM and cryostat equipment; Dr. A. Collazo and Caltech Biological Imaging Center for the training and use of confocal microscope; L. Rodriguez for administrative assistance; J. Gutierrez, K.F. Lee, J. Rodriguez, L.C. Sandoval, N.A. Verduzco for caring of animals. This work was supported by NIH Conte Center Award (NIH 5P50MH086383-04, to P.H.P.); Autism Speaks (#7670, to P.H.P); postdoctoral fellowship from National Science Council, Taiwan (NSC 101-2917-I-564-039, to W.-L.W.); Autism Speaks Weatherstone Predoctoral Fellowship (to E.Y.H.) and NIH/NRSA Predoctoral Fellowship (to E.Y.H.); Caltech Undergraduate Research Fellowship (SURF) (to Z.Y.) and Amgen Scholars Program at Caltech (to Z.Y.); the Heritage Medical Research Institute (to S.K.M.), Simons Foundation (to S.K.M.), and NIH (MH100556 to S.K.M.).
Group:Heritage Medical Research Institute
Funders:
Funding AgencyGrant Number
NIH5P50MH086383-04
Autism Speaks7670
National Science Council (Taipei)NSC 101-2917-I-564-039
NIH Predoctoral FellowshipUNSPECIFIED
Caltech Summer Undergraduate Research Fellowship (SURF)UNSPECIFIED
AmGen Scholars ProgramUNSPECIFIED
Heritage Medical Research InstituteUNSPECIFIED
Simons FoundationUNSPECIFIED
NIHMH100556
Subject Keywords:Maternal immune activation (MIA); Maternal-placental-fetal axis; Interleukin-6 (IL-6); Placenta; Hindbrain development; Autism spectrum disorder (ASD)
PubMed Central ID:PMC5373986
Record Number:CaltechAUTHORS:20161114-135126065
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20161114-135126065
Official Citation:Wei-Li Wu, Elaine Y. Hsiao, Zihao Yan, Sarkis K. Mazmanian, Paul H. Patterson, The placental interleukin-6 signaling controls fetal brain development and behavior, Brain, Behavior, and Immunity, Volume 62, May 2017, Pages 11-23, ISSN 0889-1591, https://doi.org/10.1016/j.bbi.2016.11.007. (http://www.sciencedirect.com/science/article/pii/S0889159116304974)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:71999
Collection:CaltechAUTHORS
Deposited By: Melissa Ray
Deposited On:15 Nov 2016 23:21
Last Modified:09 Mar 2020 13:19

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