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Stability of Cytoplasmic Messenger RNA in HeLa Cells

Murphy, William and Attardi, Giuseppe (1973) Stability of Cytoplasmic Messenger RNA in HeLa Cells. Proceedings of the National Academy of Sciences of the United States of America, 70 (1). pp. 115-119. ISSN 0027-8424.

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The metabolic stability of pulse-labeled or long-term labeled messenger RNA (mRNA) from cytoplasmic free polysomes was measured in HeLa cells, with chase conditions that do not involve inhibitors of RNA synthesis, and chromatography on benzoylated-DEAE-cellulose or poly(T)-cellulose for isolation of mRNA. For these studies, a new chase technique was developed that allows analysis of the stability of mRNA labeled during a short [3H]uridine pulse. Pulse-labeled and long-term labeled mRNA were found to decay with an estimated average half-life of about 2 and 3 days, respectively, much longer than hitherto assumed.

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Additional Information:Copyright © 1973 by the National Academy of Sciences Communicated by Norman Davidson, October 24, 1972 These investigations were supported by a research grant from the U.S. Public Health Service (GM-11726) and by a National Science Foundation Predoctoral Fellowship to W. M. The help of Mrs. L. Wenzel and Miss G. Engel and the excellent technical assistance of Mrs. B. Keeley are gratefully acknowledged. The poly(T)-cellulose used here was a generous gift of Dr. J. Kates.
Subject Keywords:actinomycin D, benzoylated-DEAE-cellulose, poly(T)-cellulose, nucleoside triphosphate pools, cold chase
Issue or Number:1
Record Number:CaltechAUTHORS:MURpnas73
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:720
Deposited By: Archive Administrator
Deposited On:20 Sep 2005
Last Modified:02 Oct 2019 22:36

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