A Caltech Library Service

In vivo volumetric imaging of subcutaneous microvasculature by photoacoustic microscopy

Zhang, Hao F. and Maslov, Konstantin and Li, Meng-Lin and Stoica, George and Wang, Lihong V. (2006) In vivo volumetric imaging of subcutaneous microvasculature by photoacoustic microscopy. Optics Express, 14 (20). pp. 9317-9323. ISSN 1094-4087. doi:10.1364/OE.14.009317.

[img] PDF - Published Version
See Usage Policy.


Use this Persistent URL to link to this item:


Photoacoustic microscopy was developed to achieve volumetric imaging of the anatomy and functions of the subcutaneous microvasculature in both small animals and humans in vivo with high spatial resolution and high signal-to-background ratio. By following the skin contour in raster scanning, the ultrasonic transducer maintains focusing in the region of interest. Furthermore, off-focus lateral resolution is improved by using a synthetic-aperture focusing technique based on the virtual point detector concept. Structural images are acquired in both rats and humans, whereas functional images representing hemoglobin oxygen saturation are acquired in rats. After multiscale vesselness filtering, arterioles and venules in the image are separated based on the imaged oxygen saturation levels. Detailed structural information, such as vessel depth and spatial orientation, are revealed by volume rendering.

Item Type:Article
Related URLs:
URLURL TypeDescription
Maslov, Konstantin0000-0003-3408-8840
Stoica, George0000-0001-8071-4828
Wang, Lihong V.0000-0001-9783-4383
Additional Information:© 2006 Optical Society of America. Received 20 June 2006; revised 14 August 2006; accepted 14 August 2006. We thank Dr. Geng Ku, Dr. Ovidiu Craciun, and Gina Lungu for experimental assistance. We also gratefully acknowledge the help of Dr. Yangqiu Hu in image processing. This project is sponsored by National Institutes of Health grants R01 EB000712 and R01 NS46214.
Funding AgencyGrant Number
NIHR01 EB000712
NIHR01 NS46214
Issue or Number:20
Classification Code:OCIS codes: (170.3880) Medical and biological imaging; (170.5120) Photoacoustic imaging; (180.5810) Scanning microscopy
Record Number:CaltechAUTHORS:20161118-102659762
Persistent URL:
Official Citation:Hao F. Zhang, Konstantin Maslov, Meng-Lin Li, George Stoica, and Lihong V. Wang, "In vivo volumetric imaging of subcutaneous microvasculature by photoacoustic microscopy," Opt. Express 14, 9317-9323 (2006)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:72154
Deposited By: Tony Diaz
Deposited On:18 Nov 2016 18:34
Last Modified:11 Nov 2021 04:57

Repository Staff Only: item control page