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Practical reconstruction method for bioluminescence tomography

Cong, Wenxiang and Wang, Ge and Kumar, Durairaj and Liu, Yi and Jiang, Ming and Wang, Lihong V. and Hoffman, Eric A. and McLennan, Geoffrey and McCray, Paul B. and Zabner, Joseph and Cong, Alexander (2005) Practical reconstruction method for bioluminescence tomography. Optics Express, 13 (18). pp. 6756-6771. ISSN 1094-4087. doi:10.1364/OPEX.13.006756.

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Bioluminescence tomography (BLT) is used to localize and quantify bioluminescent sources in a small living animal. By advancing bioluminescent imaging to a tomographic framework, it helps to diagnose diseases, monitor therapies and facilitate drug development. In this paper, we establish a direct linear relationship between measured surface photon density and an unknown bioluminescence source distribution by using a finite-element method based on the diffusion approximation to the photon propagation in biological tissue. We develop a novel reconstruction algorithm to recover the source distribution. This algorithm incorporates a priori knowledge to define the permissible source region in order to enhance numerical stability and efficiency. Simulations with a numerical mouse chest phantom demonstrate the feasibility of the proposed BLT algorithm and reveal its performance in terms of source location, density, and robustness against noise. Lastly, BLT experiments are performed to identify the location and power of two light sources in a physical mouse chest phantom.

Item Type:Article
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URLURL TypeDescription
Wang, Lihong V.0000-0001-9783-4383
Additional Information:© 2005 Optical Society of America. Received 11 July 2005; revised 16 August 2005; accepted 18 August 2005. This work is supported by an NIH/NIBIB grant EB001685, a special grant for bioluminescent imaging from Department of Radiology, College of Medicine, University of Iowa. Dr. Ming Jiang was also supported in part by NKBRSF (2003CB716101) and NSFC (60325101, 60272018 and 60372024), China.
Funding AgencyGrant Number
University of IowaUNSPECIFIED
National Key Basic Research Program of China2003CB716101
National Science Foundation of China60325101
National Science Foundation of China60272018
National Science Foundation of China60372024
Issue or Number:18
Classification Code:OCIS codes: (110.6960) Tomography; (170.3010) Image reconstruction techniques; (170.6280) Spectroscopy, fluorescence and luminescence
Record Number:CaltechAUTHORS:20161118-134400405
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Official Citation:Wenxiang Cong, Ge Wang, Durairaj Kumar, Yi Liu, Ming Jiang, Lihong V. Wang, Eric A. Hoffman, Geoffrey McLennan, Paul B. McCray, Joseph Zabner, and Alexander Cong, "Practical reconstruction method for bioluminescence tomography," Opt. Express 13, 6756-6771 (2005)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:72173
Deposited By: Tony Diaz
Deposited On:18 Nov 2016 22:15
Last Modified:11 Nov 2021 04:57

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