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Whorl-Specific Expression of the SUPERMAN Gene of Arabidopsis Is Mediated by cis Elements in the Transcribed Region

Ito, Toshiro and Sakai, Hajime and Meyerowitz, Elliot M. (2003) Whorl-Specific Expression of the SUPERMAN Gene of Arabidopsis Is Mediated by cis Elements in the Transcribed Region. Current Biology, 13 (17). pp. 1524-1530. ISSN 0960-9822. doi:10.1016/S0960-9822(03)00612-2. https://resolver.caltech.edu/CaltechAUTHORS:20161201-105032786

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Abstract

The SUPERMAN (SUP) gene of Arabidopsis is involved in controlling cell proliferation in stamen and carpel primordia and in ovules during flower development. The SUP gene encodes a transcription factor with a C2H2-type zinc finger motif, a serine/proline-rich domain, a basic domain, and a leucine-zipper-like domain and is expressed in a very limited region in stamen primordia and in the developing ovary during flower development. The SUP gene is susceptible to methylation, resulting in epigenetic gene silencing. To understand how the SUP gene is expressed spatially and temporally in its restricted domain, and why methylation of the transcribed region affects early-stage SUP expression, we have identified the SUP cis regulatory elements by characterizing SUP gene fusions. These studies show that the SUP gene has discrete upstream promoter elements required for expression in stamen primordia in early stages and in the ovary in later stages. The promoter activity for stamen primordia is modulated by several positive and negative elements located in the transcribed and translated regions. Several regulatory elements in the transcribed region correlate with the areas of the gene that are heavily methylated in epigenetic alleles; these data provide a possible explanation of how methylation of the transcribed region represses transcription.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/S0960-9822(03)00612-2DOIArticle
http://www.sciencedirect.com/science/article/pii/S0960982203006122PublisherArticle
ORCID:
AuthorORCID
Ito, Toshiro0000-0002-8206-2787
Meyerowitz, Elliot M.0000-0003-4798-5153
Additional Information:© 2003 Cell Press. Received: June 11, 2003. Revised: July 7, 2003. Accepted: July 9, 2003. Published: September 2, 2003. We are grateful to Steve Jacobsen (UCLA) for providing unpublished results and to Giao Hang and Natsuko Ito for their technical assistance. We thank Meyerowitz lab members, especially Hao Yu, Annick Dubios, Elizabeth Haswell, Pradeep Das, and Ioan Negrutiu for valuable comments on the manuscript. This work was supported by National Science Foundation Grant IBN-0211670.
Funders:
Funding AgencyGrant Number
NSFIBN-0211670
Issue or Number:17
DOI:10.1016/S0960-9822(03)00612-2
Record Number:CaltechAUTHORS:20161201-105032786
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20161201-105032786
Official Citation:Toshiro Ito, Hajime Sakai, Elliot M. Meyerowitz, Whorl-Specific Expression of the SUPERMAN Gene of Arabidopsis Is Mediated by cis Elements in the Transcribed Region, Current Biology, Volume 13, Issue 17, 2 September 2003, Pages 1524-1530, ISSN 0960-9822, http://dx.doi.org/10.1016/S0960-9822(03)00612-2. (http://www.sciencedirect.com/science/article/pii/S0960982203006122)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:72492
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:01 Dec 2016 19:23
Last Modified:11 Nov 2021 05:02

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