CaltechAUTHORS
  A Caltech Library Service

Patterns of Auxin Transport and Gene Expression during Primordium Development Revealed by Live Imaging of the Arabidopsis Inflorescence Meristem

Heisler, Marcus G. and Ohno, Carolyn and Das, Pradeep and Sieber, Patrick and Reddy, Gonehal V. and Long, Jeff A. and Meyerowitz, Elliot M. (2005) Patterns of Auxin Transport and Gene Expression during Primordium Development Revealed by Live Imaging of the Arabidopsis Inflorescence Meristem. Current Biology, 15 (21). pp. 1899-1911. ISSN 0960-9822. http://resolver.caltech.edu/CaltechAUTHORS:20161201-113508748

[img] PDF (Document S1. Two figures) - Supplemental Material
See Usage Policy.

177Kb
[img] Video (QuickTime) (Movie S1. PIN1-GFP Expression Dynamics) - Supplemental Material
See Usage Policy.

1729Kb
[img] Video (QuickTime) (Movie S2. PIN1-GFP Polarity Reversal Dynamics) - Supplemental Material
See Usage Policy.

9Mb

Use this Persistent URL to link to this item: http://resolver.caltech.edu/CaltechAUTHORS:20161201-113508748

Abstract

Background: Plants produce leaf and flower primordia from a specialized tissue called the shoot apical meristem (SAM). Genetic studies have identified a large number of genes that affect various aspects of primordium development including positioning, growth, and differentiation. So far, however, a detailed understanding of the spatio-temporal sequence of events leading to primordium development has not been established. Results: We use confocal imaging of green fluorescent protein (GFP) reporter genes in living plants to monitor the expression patterns of multiple proteins and genes involved in flower primordial developmental processes. By monitoring the expression and polarity of PINFORMED1 (PIN1), the auxin efflux facilitator, and the expression of the auxin-responsive reporter DR5, we reveal stereotypical PIN1 polarity changes which, together with auxin induction experiments, suggest that cycles of auxin build-up and depletion accompany, and may direct, different stages of primordium development. Imaging of multiple GFP-protein fusions shows that these dynamics also correlate with the specification of primordial boundary domains, organ polarity axes, and the sites of floral meristem initiation. Conclusions: These results provide new insight into auxin transport dynamics during primordial positioning and suggest a role for auxin transport in influencing primordial cell type.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/j.cub.2005.09.052DOIArticle
http://www.sciencedirect.com/science/article/pii/S0960982205012133PublisherArticle
ORCID:
AuthorORCID
Heisler, Marcus G.0000-0001-5644-8398
Meyerowitz, Elliot M.0000-0003-4798-5153
Additional Information:© 2005 Elsevier Ltd. Received: August 11, 2005. Revised: September 27, 2005. Accepted: September 27, 2005. Published: November 7, 2005. We are grateful to members of the Meyerowitz laboratory for critical comments and suggestions for the manuscript. We thank Jan Traas for communicating unpublished results. This work was supported by Department of Energy grant DOE FG02-88ER13873 and National Science Foundation grant FIBR 0330786.
Funders:
Funding AgencyGrant Number
Department of Energy (DOE)DE-FG02-88ER13873
NSFFIBR-0330786
Record Number:CaltechAUTHORS:20161201-113508748
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20161201-113508748
Official Citation:Marcus G. Heisler, Carolyn Ohno, Pradeep Das, Patrick Sieber, Gonehal V. Reddy, Jeff A. Long, Elliot M. Meyerowitz, Patterns of Auxin Transport and Gene Expression during Primordium Development Revealed by Live Imaging of the Arabidopsis Inflorescence Meristem, Current Biology, Volume 15, Issue 21, 8 November 2005, Pages 1899-1911, ISSN 0960-9822, http://dx.doi.org/10.1016/j.cub.2005.09.052. (http://www.sciencedirect.com/science/article/pii/S0960982205012133)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:72494
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:01 Dec 2016 20:53
Last Modified:19 Sep 2017 17:19

Repository Staff Only: item control page