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Antagonistic Regulation of PIN Phosphorylation by PP2A and PINOID Directs Auxin Flux

Michniewicz, Marta and Zago, Marcelo K. and Abas, Lindy and Weijers, Dolf and Schweighofer, Alois and Meskiene, Irute and Heisler, Marcus G. and Ohno, Carolyn and Zhang, Jing and Huang, Fang and Schwab, Rebecca and Weigel, Detlef and Meyerowitz, Elliot M. and Luschnig, Christian and Offringa, Remko and Friml, Jiří (2007) Antagonistic Regulation of PIN Phosphorylation by PP2A and PINOID Directs Auxin Flux. Cell, 130 (6). pp. 1044-1056. ISSN 0092-8674. http://resolver.caltech.edu/CaltechAUTHORS:20161201-121147704

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Abstract

In plants, cell polarity and tissue patterning are connected by intercellular flow of the phytohormone auxin, whose directional signaling depends on polar subcellular localization of PIN auxin transport proteins. The mechanism of polar targeting of PINs or other cargos in plants is largely unidentified, with the PINOID kinase being the only known molecular component. Here, we identify PP2A phosphatase as an important regulator of PIN apical-basal targeting and auxin distribution. Genetic analysis, localization, and phosphorylation studies demonstrate that PP2A and PINOID both partially colocalize with PINs and act antagonistically on the phosphorylation state of their central hydrophilic loop, hence mediating PIN apical-basal polar targeting. Thus, in plants, polar sorting by the reversible phosphorylation of cargos allows for their conditional delivery to specific intracellular destinations. In the case of PIN proteins, this mechanism enables switches in the direction of intercellular auxin fluxes, which mediate differential growth, tissue patterning, and organogenesis.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/j.cell.2007.07.033DOIArticle
http://www.sciencedirect.com/science/article/pii/S0092867407009713PublisherArticle
ORCID:
AuthorORCID
Heisler, Marcus G.0000-0001-5644-8398
Meyerowitz, Elliot M.0000-0003-4798-5153
Additional Information:© 2007 Elsevier Inc. Received: August 9, 2006. Revised: April 6, 2007. Accepted: July 23, 2007. Published: September 20, 2007. We thank S. Boeren, R. Karlova, J. Kleine-Vehn, F. Maraschin and H. Robert for technical help and advice; S. Peck for helpful discussions; J. Strauss and L. Mach for sharing reagents. We gratefully acknowledge financial support from the VolkswagenStiftung and EMBO Young Investigator Program (J.F.), the Deutsche Forschungsgemeinschaft - SFB 446 (M.M.), Brazilian Funding Agency for Post-Graduation Education-CAPES (M.K.Z.), the Austrian Research Fund - P16311; P18440 (L.A., C.L.), - P16409; P19005 (A.S., I.M.), the Netherlands Proteomic Center (NPC; Grant to S. de Vries; D.W.), the China Scholarship Council (F.H.), the National Science Foundation FIBR 0330786 and Department of Energy FG02-88ER13873 (M.G.H., C.O. and E.M.M.).
Funders:
Funding AgencyGrant Number
Volkswagen StiftungUNSPECIFIED
Deutsche Forschungsgemeinschaft (DFG)SFB 446
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)UNSPECIFIED
FWF Der WissenschaftsfondsP16311
FWF Der WissenschaftsfondsP18440
FWF Der WissenschaftsfondsP16409
FWF Der WissenschaftsfondsP19005
Netherlands Proteomic CenterUNSPECIFIED
China Scholarship CouncilUNSPECIFIED
NSFFIBR-0330786
Department of Energy (DOE)FG02-88ER13873
European Molecular Biology Organization (EMBO)UNSPECIFIED
Subject Keywords:CELLBIO; SIGNALING
Record Number:CaltechAUTHORS:20161201-121147704
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20161201-121147704
Official Citation:Marta Michniewicz, Marcelo K. Zago, Lindy Abas, Dolf Weijers, Alois Schweighofer, Irute Meskiene, Marcus G. Heisler, Carolyn Ohno, Jing Zhang, Fang Huang, Rebecca Schwab, Detlef Weigel, Elliot M. Meyerowitz, Christian Luschnig, Remko Offringa, Jiří Friml, Antagonistic Regulation of PIN Phosphorylation by PP2A and PINOID Directs Auxin Flux, Cell, Volume 130, Issue 6, 21 September 2007, Pages 1044-1056, ISSN 0092-8674, http://dx.doi.org/10.1016/j.cell.2007.07.033. (http://www.sciencedirect.com/science/article/pii/S0092867407009713)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:72495
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:01 Dec 2016 20:40
Last Modified:19 Sep 2017 17:15

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