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Two MscS Homologs Provide Mechanosensitive Channel Activities in the Arabidopsis Root

Haswell, Elizabeth S. and Peyronnet, Rémi and Barbier-Brygoo, Hélène and Meyerowitz, Elliot M. and Frachisse, Jean-Marie (2008) Two MscS Homologs Provide Mechanosensitive Channel Activities in the Arabidopsis Root. Current Biology, 18 (10). pp. 730-734. ISSN 0960-9822. doi:10.1016/j.cub.2008.04.039. https://resolver.caltech.edu/CaltechAUTHORS:20161201-152903534

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Abstract

In bacterial and animal systems, mechanosensitive (MS) ion channels are thought to mediate the perception of pressure, touch, and sound 1, 2 and 3. Although plants respond to a wide variety of mechanical stimuli, and although many mechanosensitive channel activities have been characterized in plant membranes by the patch-clamp method, the molecular nature of mechanoperception in plant systems has remained elusive [4]. Likely candidates are relatives of MscS (Mechanosensitive channel of small conductance), a well-characterized MS channel that serves to protect E. coli from osmotic shock [5]. Ten MscS-Like (MSL) proteins are found in the genome of the model flowering plant Arabidopsis thaliana 4, 6 and 7. MSL2 and MSL3, along with MSC1, a MscS family member from green algae, are implicated in the control of organelle morphology 8 and 9. Here, we characterize MSL9 and MSL10, two MSL proteins found in the plasma membrane of root cells. We use a combined genetic and electrophysiological approach to show that MSL9 and MSL10, along with three other members of the MSL family, are required for MS channel activities detected in protoplasts derived from root cells. This is the first molecular identification and characterization of MS channels in plant membranes.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/j.cub.2008.04.039DOIArticle
http://www.sciencedirect.com/science/article/pii/S0960982208005253PublisherArticle
ORCID:
AuthorORCID
Haswell, Elizabeth S.0000-0002-4246-065X
Meyerowitz, Elliot M.0000-0003-4798-5153
Additional Information:© 2008 Elsevier Ltd. Received: February 20, 2008. Revised: April 9, 2008. Accepted: April 11, 2008. Published online: May 15, 2008. This work was funded by the French Ministry of Research, the Centre National de la Recherche Scientifique, and the U.S. Department of Energy (grant DE-FG02-88ER13873). We thank the Salk Institute Genomic Analysis Laboratory for providing the sequence-indexed Arabidopsis T-DNA insertion mutants used in this study.
Funders:
Funding AgencyGrant Number
Ministère de l’Enseignement supérieur et de la RechercheUNSPECIFIED
Centre National de la Recherche Scientifique (CNRS)UNSPECIFIED
Department of Energy (DOE)DE-FG02-88ER13873
Subject Keywords:CELLBIO
Issue or Number:10
DOI:10.1016/j.cub.2008.04.039
Record Number:CaltechAUTHORS:20161201-152903534
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20161201-152903534
Official Citation:Elizabeth S. Haswell, Rémi Peyronnet, Hélène Barbier-Brygoo, Elliot M. Meyerowitz, Jean-Marie Frachisse, Two MscS Homologs Provide Mechanosensitive Channel Activities in the Arabidopsis Root, Current Biology, Volume 18, Issue 10, 20 May 2008, Pages 730-734, ISSN 0960-9822, http://dx.doi.org/10.1016/j.cub.2008.04.039. (http://www.sciencedirect.com/science/article/pii/S0960982208005253)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:72506
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:02 Dec 2016 23:18
Last Modified:11 Nov 2021 05:02

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