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Genome-Wide Analysis of Spatial Gene Expression in Arabidopsis Flowers

Wellmer, Frank and Riechmann, José Luis and Alves-Ferreira, Márcio and Meyerowitz, Elliot M. (2004) Genome-Wide Analysis of Spatial Gene Expression in Arabidopsis Flowers. Plant Cell, 16 (5). pp. 1314-1326. ISSN 1040-4651. PMCID PMC423218. https://resolver.caltech.edu/CaltechAUTHORS:20161202-064723249

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Abstract

We have compared the gene expression profiles of inflorescences of the floral homeotic mutants apetala1, apetala2, apetala3, pistillata, and agamous with that of wild-type plants using a flower-specific cDNA microarray and a whole genome oligonucleotide array. By combining the data sets from the individual mutant/wild type comparisons, we were able to identify a large number of genes that are, within flowers, predicted to be specifically or at least predominantly expressed in one type of floral organ. We have analyzed the expression patterns of several of these genes by in situ hybridization and found that they match the predictions that were made based on the microarray experiments. Moreover, genes with known floral organ–specific expression patterns were correctly assigned by our analysis. The vast majority of the identified transcripts are found in stamens or carpels, whereas few genes are predicted to be expressed specifically or predominantly in sepals or petals. These findings indicate that spatially limited expression of a large number of genes is part of flower development and that its extent differs significantly between the reproductive organs and the organs of the perianth.


Item Type:Article
Related URLs:
URLURL TypeDescription
http:/​/​dx.​doi.​org/​10.​1105/​tpc.​021741DOIArticle
http://www.plantcell.org/content/16/5/1314.shortPublisherArticle
http://www.plantcell.org/content/16/5/1314/suppl/DC1PublisherSupplemental Data
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC423218/PubMed CentralArticle
ORCID:
AuthorORCID
Meyerowitz, Elliot M.0000-0003-4798-5153
Additional Information:© 2004 by the American Society of Plant Biologists. Received February 11, 2004. Accepted February 12, 2004. Published April 20, 2004. We are very grateful to Vijaya Rao and Ali Mortazavi for help with microarray data analysis, to Arnavaz Garda for technical assistance, and to members of the Meyerowitz laboratory for discussion and for comments on the manuscript. We thank S. Tabata (Kazusa DNA Research Institute, Japan) for the generous gift of the flower-derived EST collection. This work was supported by National Institutes of Health Grant GM45697 to E.M.M. and by the Millard and Muriel Jacobs Genetics and Genomics Laboratory at California Institute of Technology. F.W. was supported by an Emmy Noether fellowship of the Deutsche Forschungsgemeinschaft. M.A.-F. is indebted to the Instituut voor Plantenbiotechnologie voor Ontwikkelingslanden and Marc Van Montagu for a fellowship sponsored by Aventis Crop Sciences.
Funders:
Funding AgencyGrant Number
NIHGM45697
Caltech Millard and Muriel Jacobs Genetics and Genomics LaboratoryUNSPECIFIED
Deutsche Forschungsgemeinschaft (DFG)UNSPECIFIED
Aventis Crop SciencesUNSPECIFIED
Issue or Number:5
PubMed Central ID:PMC423218
Record Number:CaltechAUTHORS:20161202-064723249
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20161202-064723249
Official Citation:Genome-Wide Analysis of Spatial Gene Expression in Arabidopsis Flowers Frank Wellmer, José Luis Riechmann, Márcio Alves-Ferreira and Elliot M. Meyerowitz Advance Publication April 20, 2004, doi: http:/​/​dx.​doi.​org/​10.​1105/​tpc.​021741 The Plant Cell May 2004 vol. 16 no. 5 1314-1326
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:72509
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:02 Dec 2016 23:09
Last Modified:03 Oct 2019 16:18

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