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DNA sequences of the repressor gene and operator region of bacteriophage P2

Ljungquist, Elisabeth and Kockum, Kerstin and Bertani, L. Elizabeth (1984) DNA sequences of the repressor gene and operator region of bacteriophage P2. Proceedings of the National Academy of Sciences of the United States of America, 81 (13). pp. 3988-3992. ISSN 0027-8424. PMCID PMC345353. doi:10.1073/pnas.81.13.3988.

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The nucleotide sequence of the repressor gene C of the temperate phage P2 has been determined. It codes for a nonbasic polypeptide, 99 amino acids long. Twelve repressor-defective mutants have been mapped. All but one are located within the presumed coding part of the gene. There is a strong promoter sequence and an 8-base-pair inverted repeat preceding the gene. The P2 repressor protein shows structural similarity to other DNA-binding proteins. The operator region for the early replication functions was located by sequencing the DNA of three virulent mutants. The sequence indicates that there are two repressor-binding sites. In addition, one of the sites shows sequence homology with part of the operator region of the biotin operon of Escherichia coli.

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Additional Information:© 1984 by the National Academy of Sciences. Communicated by Norman H. Horowitz, March 12, 1984. We would like to thank Peter Alestrom for advice on the DNA sequencing and G. Bertani for helpful discussions. A large part of the DNA sequencing was done in the Department of Microbiology at the University of Uppsala, Uppsala, Sweden. This work was supported by Grant 72 from the Swedish Medical Research Council. The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.
Funding AgencyGrant Number
Swedish Medical Research Council72
Subject Keywords:overlapping transcription; DNA-binding proteins; biotin operator
Issue or Number:13
PubMed Central ID:PMC345353
Record Number:CaltechAUTHORS:LJUpnas84
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:7254
Deposited By: Tony Diaz
Deposited On:24 Jan 2007
Last Modified:08 Nov 2021 20:40

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