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Global Expression Profiling Applied to the Analysis of Arabidopsis Stamen Development

Alves-Ferreira, Márcio and Wellmer, Frank and Banhara, Aline and Kumar, Vijaya and Riechmann, José Luis and Meyerowitz, Elliot M. (2007) Global Expression Profiling Applied to the Analysis of Arabidopsis Stamen Development. Plant Physiology, 145 (3). pp. 747-762. ISSN 0032-0889. PMCID PMC2048804. https://resolver.caltech.edu/CaltechAUTHORS:20161205-105621673

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Abstract

To obtain detailed information about gene expression during stamen development in Arabidopsis (Arabidopsis thaliana), we compared, by microarray analysis, the gene expression profile of wild-type inflorescences to those of the floral mutants apetala3, sporocyteless/nozzle, and male sterile1 (ms1), in which different aspects of stamen formation are disrupted. These experiments led to the identification of groups of genes with predicted expression at early, intermediate, and late stages of stamen development. Validation experiments using in situ hybridization confirmed the predicted expression patterns. Additional experiments aimed at characterizing gene expression specifically during microspore formation. To this end, we compared the gene expression profiles of wild-type flowers of distinct developmental stages to those of the ms1 mutant. Computational analysis of the datasets derived from this experiment led to the identification of genes that are likely involved in the control of key developmental processes during microsporogenesis. We also identified a large number of genes whose expression is prolonged in ms1 mutant flowers compared to the wild type. This result suggests that MS1, which encodes a putative transcriptional regulator, is involved in the stage-specific repression of these genes. Lastly, we applied reverse genetics to characterize several of the genes identified in the microarray experiments and uncovered novel regulators of microsporogenesis, including the transcription factor MYB99 and a putative phosphatidylinositol 4-kinase.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://​dx.​doi.​org/​10.​1104/​pp.​107.​104422DOIArticle
http://www.plantphysiol.org/content/145/3/747PublisherArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2048804PubMed CentralArticle
http://www.plantphysiol.org/content/145/3/747/suppl/DC1PublisherSupplemental Data
ORCID:
AuthorORCID
Meyerowitz, Elliot M.0000-0003-4798-5153
Additional Information:© 2007 American Society of Plant Biologists. Received June 21, 2007. Accepted September 14, 2007. Published September 28, 2007. This work was supported by the National Institutes of Health (grant no. GM45697 to E.M.M.), the Millard and Muriel Jacobs Genetics and Genomics Laboratory at the California Institute of Technology, the Conselho Nacional de Desenvolvimento Científico e Tecnológico (grant nos. 307219/2004-6, 400767/2004-0, and 475666/2004-6 to M.A.-F. and fellowship to A.B.), the Fundacxão Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro (grant no. E-26/171.332/2004 to M.A.-F. and fellowship to A.B.), the International Foundation for Science (grant no. C/3962-1 to M.A.-F.), the International Basic Sciences Programme (grant no. IBSP/UNESCO-3–BR-28 to M.A.-F.), and Aventis Crop Sciences (fellowship to M.A.-F.). We thank Arnavaz Garda and Beatriz Dias for technical assistance.
Funders:
Funding AgencyGrant Number
NIHGM45697
Caltech Millard and Muriel Jacobs Genetics and Genomics LaboratoryUNSPECIFIED
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)307219/2004–6
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)400767/2004–0
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)475666/2004–6
Fundacxão Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de JaneiroE–26/171.332/2004
International Foundation for ScienceC/3962-1
International Basic Sciences ProgrammeIBSP/UNESCO-3-BR-28
Aventis Crop SciencesUNSPECIFIED
Issue or Number:3
PubMed Central ID:PMC2048804
Record Number:CaltechAUTHORS:20161205-105621673
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20161205-105621673
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:72554
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:07 Dec 2016 00:18
Last Modified:03 Oct 2019 16:19

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