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Microarray analysis reveals altered expression of a large number of nuclear genes in developing cytoplasmic male sterile Brassica napus flowers

Carlsson, Jenny and Lagercrantz, Ulf and Sundström, Jens and Teixeira, Rita and Wellmer, Frank and Meyerowitz, Elliot M. and Glimelius, Kristina (2007) Microarray analysis reveals altered expression of a large number of nuclear genes in developing cytoplasmic male sterile Brassica napus flowers. Plant Journal, 49 (3). pp. 452-462. ISSN 0960-7412. https://resolver.caltech.edu/CaltechAUTHORS:20161206-082713761

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Abstract

To gain new insights into the mechanism underlying cytoplasmic male sterility (CMS), we compared the nuclear gene expression profiles of flowers of a Brassica napus CMS line with that of the fertile B. napus maintainer line using Arabidopsis thaliana flower-specific cDNA microarrays. The CMS line used has a B. napus nuclear genome, but has a rearranged mitochondrial (mt) genome consisting of both B. napus and A. thaliana DNA. Gene expression profiling revealed that a large number of genes differed in expression between the two lines. For example, nuclear genes coding for proteins that are involved in protein import into organelles, genes expressed in stamens and pollen, as well as genes implicated in either cell-wall remodeling or architecture, were repressed in the CMS line compared with B. napus. These results show that the mt genome of the CMS line strongly influences nuclear gene expression, and thus reveal the importance of retrograde signalling between the mitochondria and the nucleus. Furthermore, flowers of the CMS line are characterized by a replacement of stamens with carpelloid organs, and thus partially resemble the APETALA3 (AP3) and PISTILLATA (PI) mutants. In accordance with this phenotype, AP3 expression was downregulated in the stamens, shortly before these organs developed carpelloid characteristics, even though it was initiated correctly. Repression of PI succeeded that of AP3 and might be a consequence of a loss of AP3 activity. These results suggest that AP3 expression in stamens depends on proper mt function and a correct nuclear–mt interaction, and that mt alterations cause the male sterility phenotype of the CMS line.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1111/j.1365-313X.2006.02975.xDOIArticle
http://onlinelibrary.wiley.com/doi/10.1111/j.1365-313X.2006.02975.x/abstractPublisherArticle
ORCID:
AuthorORCID
Meyerowitz, Elliot M.0000-0003-4798-5153
Additional Information:© 2007 The Authors. Journal compilation © 2007 Blackwell Publishing Ltd. Received 17 September 2006; accepted 26 September 2006. We thank I. Eriksson, G. Rönnqvist and S. Thyselius for their excellent technical assistance and Dr M. Leino for valuable discussions. This work was supported by the strategic research programme Agricultural Functional Genomics (AgriFunGen) at the Swedish University of Agricultural Sciences, as well as The Swedish Research Council (VR), The Swedish Research Council for Environment, Agricultural Sciences and Spatial Planning (FORMAS) and The Swedish Foundation for International Cooperation in Research and Higher Education (STINT).
Funders:
Funding AgencyGrant Number
Swedish University of Agricultural SciencesUNSPECIFIED
Swedish Research CouncilUNSPECIFIED
Swedish Foundation for International Cooperation in Research and Higher Education (STINT)UNSPECIFIED
Subject Keywords:cytoplasmic male sterility; flower development; rearranged mitochondrial DNA; Brassica napus; Arabidopsis thaliana; microarray
Issue or Number:3
Record Number:CaltechAUTHORS:20161206-082713761
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20161206-082713761
Official Citation:Carlsson, J., Lagercrantz, U., Sundström, J., Teixeira, R., Wellmer, F., Meyerowitz, E. M. and Glimelius, K. (2007), Microarray analysis reveals altered expression of a large number of nuclear genes in developing cytoplasmic male sterile Brassica napus flowers. The Plant Journal, 49: 452–462. doi:10.1111/j.1365-313X.2006.02975.x
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:72587
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:06 Dec 2016 23:46
Last Modified:03 Oct 2019 16:19

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