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The Homeotic Protein AGAMOUS Controls Late Stamen Development by Regulating a Jasmonate Biosynthetic Gene in Arabidopsis

Ito, Toshiro and Ng, Kian-Hong and Lim, Tze-Soo and Yu, Hao and Meyerowitz, Elliot M. (2007) The Homeotic Protein AGAMOUS Controls Late Stamen Development by Regulating a Jasmonate Biosynthetic Gene in Arabidopsis. Plant Cell, 19 (11). pp. 3516-3529. ISSN 1040-4651. PMCID PMC2174883. https://resolver.caltech.edu/CaltechAUTHORS:20161206-112140417

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Abstract

The Arabidopsis thaliana floral homeotic gene AGAMOUS (AG) plays a central role in reproductive organ (stamen and carpel) development. AG RNA is expressed in the center of floral primordia from a time prior to the initiation of stamen and carpel primordia until late in flower development. While early AG expression acts in specification of stamens and carpels, the role, if any, of continued AG expression in later flower development is unknown. To examine the timing of AG action and its possible late-stage functions, we performed a series of time-course experiments using a transgenic line with inducible AG activity in an ag homozygous mutant background. We show that AG controls late-stage stamen development, including anther morphogenesis and dehiscence, as well as filament formation and elongation. We further show that AG coordinates late stamen maturation by controlling a biosynthetic gene of the lipid-derived phytohormone jasmonic acid (JA). Expression analysis and in vivo binding of AG indicate that AG directly regulates the transcription of a catalytic enzyme of JA, DEFECTIVE IN ANTHER DEHISCENCE1. Our results indicate that stamen identity and differentiation control by AG is achieved by the regulation of different transcriptional cascades in different floral stages, with organ specification induced early, followed by phytohormone biosynthesis to coordinate stamen maturation.


Item Type:Article
Related URLs:
URLURL TypeDescription
http:/​/​dx.​doi.​org/​10.​1105/​tpc.​107.​055467DOIArticle
http://www.plantcell.org/content/19/11/3516.abstractPublisherArticle
http://www.plantcell.org/content/19/11/3516/suppl/DC1PublisherSupplemental Data
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2174883/PubMed CentralArticle
ORCID:
AuthorORCID
Ito, Toshiro0000-0002-8206-2787
Meyerowitz, Elliot M.0000-0003-4798-5153
Additional Information:© 2007 American Society of Plant Biologists. Received September 1, 2007. Revised October 7, 2007. Accepted October 10, 2007. Published November 2, 2007. We thank E.M.M.'s lab members Nicole Kubat, Jerry Guo, and Ransom Poythress and T.I.'s lab members Zhan Dan and Natsuko Ito for their diligent help with the experiments. We also thank Motomi Ito (University of Tokyo, Japan) and Mitsuyasu Hasebe (National Institute for Basic Biology, Japan) for personal communication, Pernille Rorth (Temasek Life Sciences Laboratory), former Meyerowitz lab members M. Frohlich (The Natural History Museum, London, UK), P. Kumar (National University of Singapore), G. Venugopala Reddy (University of California, Riverside), and current lab members Adrienne Roeder, Elizabeth Haswell, and Carolyn Ohno for valuable comments on the manuscript. This work was partially supported by Grant GM45697 from the National Institutes of Health to E.M.M. and by a research grant to the Temasek Life Sciences Laboratory to T.I.
Funders:
Funding AgencyGrant Number
NIHGM45697
Temasek Life Sciences LaboratoryUNSPECIFIED
Issue or Number:11
PubMed Central ID:PMC2174883
Record Number:CaltechAUTHORS:20161206-112140417
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20161206-112140417
Official Citation:Toshiro Ito, Kian-Hong Ng, Tze-Soo Lim, Hao Yu, and Elliot M. Meyerowitz The Homeotic Protein AGAMOUS Controls Late Stamen Development by Regulating a Jasmonate Biosynthetic Gene in Arabidopsis Plant Cell November 2007 19: 3516-3529. Advance Publication November 2, 2007; doi:10.1105/tpc.107.055467
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:72596
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:06 Dec 2016 23:25
Last Modified:09 Mar 2020 13:18

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