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Noninvasive photoacoustic angiography of animal brains in vivo with near-infrared light and an optical contrast agent

Wang, Xueding and Ku, Geng and Wegiel, Malgorzata A. and Bornhop, Darryl J. and Stoica, George and Wang, Lihong V. (2004) Noninvasive photoacoustic angiography of animal brains in vivo with near-infrared light and an optical contrast agent. Optics Letters, 29 (7). pp. 730-732. ISSN 0146-9592. doi:10.1364/OL.29.000730. https://resolver.caltech.edu/CaltechAUTHORS:20161207-150017901

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Abstract

Optical contrast agents have been widely applied to enhance the sensitivity and specificity of optical imaging with near-infrared (NIR) light. However, because of the overwhelming scattering of light in biological tissues, the spatial resolution of traditional optical imaging degrades drastically as the imaging depth increases. Here, for the first time to our knowledge, we present noninvasive photoacoustic angiography of animal brains in vivo with NIR light and an optical contrast agent. When indocyanine green polyethylene glycol, a novel absorption dye with prolonged clearance, is injected into the circulatory system of a rat, it obviously enhances the absorption contrast between the blood vessels and the background tissues. Because NIR light can penetrate deep into the brain tissues through the skin and skull, we are able to successfully reconstruct the vascular distribution in the rat brain from the photoacoustic signals. On the basis of differential optical absorption with and without contrast enhancement, a photoacoustic angiograph of a rat brain is acquired that matches the anatomical photograph well and exhibits high spatial resolution and a much-reduced background. This new technology demonstrates the potential for dynamic and molecular biomedical imaging.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1364/OL.29.000730DOIArticle
https://www.osapublishing.org/ol/abstract.cfm?uri=ol-29-7-730PublisherArticle
ORCID:
AuthorORCID
Stoica, George0000-0001-8071-4828
Wang, Lihong V.0000-0001-9783-4383
Additional Information:© 2004 Optical Society of America. Received August 21, 2003. This study was sponsored in part by the National Institutes of Health, the Department of Defense, the National Science Foundation, and the Texas Advanced Research Program.
Funders:
Funding AgencyGrant Number
NIHUNSPECIFIED
Department of DefenseUNSPECIFIED
NSFUNSPECIFIED
Texas Advanced Research ProgramUNSPECIFIED
Issue or Number:7
Classification Code:OCIS codes: 170.5120, 170.3880, 170.0110, 170.1470
DOI:10.1364/OL.29.000730
Record Number:CaltechAUTHORS:20161207-150017901
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20161207-150017901
Official Citation:Xueding Wang, Geng Ku, Malgorzata A. Wegiel, Darryl J. Bornhop, George Stoica, and Lihong V. Wang, "Noninvasive photoacoustic angiography of animal brains in vivo with near-infrared light and an optical contrast agent," Opt. Lett. 29, 730-732 (2004)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:72640
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:07 Dec 2016 23:14
Last Modified:11 Nov 2021 05:04

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