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Overexpression of a Gene Encoding a Cytochrome P450, CYP78A9, Induces Large and Seedless Fruit in Arabidopsis

Ito, Toshiro and Meyerowitz, Elliot M. (2000) Overexpression of a Gene Encoding a Cytochrome P450, CYP78A9, Induces Large and Seedless Fruit in Arabidopsis. Plant Cell, 12 (9). pp. 1541-1550. ISSN 1040-4651. PMCID PMC149068. doi:10.1105/tpc.12.9.1541.

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An activation tagging screen in which the cauliflower mosaic virus 35S enhancer was inserted randomly into an Arabidopsis genome homozygous for the floral homeotic mutation apetala2-1 (ap2-1) resulted in a line (28-5) with extraordinarily wide, heart-shaped ovaries. The ovary of the 28-5 ap2-1 mutant shows an oval shape because of increased numbers of enlarged cells. When the ap2-1 mutation is crossed out of the genetic background, more elongated rather than wider fruits are obtained. Normally, Arabidopsis fruits will develop to a normal size only when the ovules are present and fertilized. In the 28-5 single mutant, the siliques keep growing despite failure of fertilization and can reach nearly normal size. When wild-type pollen was used to pollinate the mutant pistil, the pollinated 28-5 silique became >10% longer and 40% wider than a wild-type silique, although producing very few seeds. The enhancer insertion in line 28-5 acts by hyperactivating a cytochrome P450 gene, CYP78A9. The pistil of 28-5 ap2-1 mutant flowers shows a structure similar to that of Capsella bursa-pastoris, a distant mustard relative of Arabidopsis, suggesting that the processes regulated by the CYP78A9-encoded protein may be involved in evolutionary control of carpel shape.

Item Type:Article
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URLURL TypeDescription CentralArticle
Ito, Toshiro0000-0002-8206-2787
Meyerowitz, Elliot M.0000-0003-4798-5153
Additional Information:© 2000 American Society of Plant Physiologists. Received April 12, 2000; accepted June 20, 2000. We thank Detlef Weigel (The Salk Institute for Biological Studies, La Jolla, CA) for supplying the activation tagging vector pSKI015. We are grateful to Caroline Lim, Marjorie L. James, and Giao Hang for their help with making transgenic plants and their molecular analysis. We also thank Caroline Lim, Carolyn Ohno, Catherine Baker, Chiou-Fen Chuang, Doris Wagner, Eva Ziegelhoffer, Frank Wellmer, Giao Hang, Michael Vishnevetsky, and Venugopala Reddy Gonehal for critical reading of the manuscript. This work was supported by National Institutes of Health Grant No. GM45697 to E.M.M. T.I. was supported by a Japan Society for the Promotion of Science (JSPS) Postdoctoral Fellowship for Research Abroad from 1997 through 1999.
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Japan Society for the Promotion of Science (JSPS)UNSPECIFIED
Issue or Number:9
PubMed Central ID:PMC149068
Record Number:CaltechAUTHORS:20161212-155724041
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:72737
Deposited By: George Porter
Deposited On:13 Dec 2016 02:29
Last Modified:11 Nov 2021 05:05

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