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A new high-capacity cosmid vector and its use

Meyerowitz, Elliot M. and Guild, Gregory M. and Prestidge, Louise S. and Hogness, David S. (1980) A new high-capacity cosmid vector and its use. Gene, 11 (3-4). pp. 271-282. ISSN 0378-1119. doi:10.1016/0378-1119(80)90067-0. https://resolver.caltech.edu/CaltechAUTHORS:20161212-163941771

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Abstract

The construction of a cosmid, MUA-3, designed for the convenient cloning of eukaryotic DNA segments up to 48 kb in length is described. The cosmid contains all of the plasmid pBR322 with approx. 400 bases of λ DNA, including the cohesive end site, inserted at the pBR322 PstI endonuclease recognition site. Methods for using this vector to construct several types of Drosophila melanogaster genomic DNA libraries are given, and libraries made by these methods are characterized. A sheared Drosophila DNA-EcoRI linker library is shown to stably maintain average Drosophila DNA inserts of over 40 kb and up to 48 kb, and the efficiency of producing clones by a partial restriction and ligation method is shown to be over 3 × 10^5 clones/μg of Drosophila DNA.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/0378-1119(80)90067-0DOIArticle
http://www.sciencedirect.com/science/article/pii/0378111980900670PublisherArticle
ORCID:
AuthorORCID
Meyerowitz, Elliot M.0000-0003-4798-5153
Additional Information:© 1980 Elsevier. Received 13 April 1980, Accepted 25 June 1980. Communicated by J. Carbon. This work was supported by research grants from the National Science Foundation, the National Institute of Health and the American Cancer Society. E.M.M. and G.M.G. were fellows of the Jane Coffin Childs Memorial Fund for Medical Research, G.M.G. was also supported in part by the American Cancer Society - California Division.
Funders:
Funding AgencyGrant Number
NSFUNSPECIFIED
NIHUNSPECIFIED
American Cancer SocietyUNSPECIFIED
Jane Coffin Childs Memorial Fund for Medical ResearchUNSPECIFIED
American Cancer Society, California DivisionUNSPECIFIED
Subject Keywords:Recombinant DNA; Drosophila melanogaster; genomic libraries; bacteriophage λ
Issue or Number:3-4
DOI:10.1016/0378-1119(80)90067-0
Record Number:CaltechAUTHORS:20161212-163941771
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20161212-163941771
Official Citation:Elliot M. Meyerowitz, Gregory M. Guild, Louise S. Prestidge, David S. Hogness, A new high-capacity cosmid vector and its use, Gene, Volume 11, Issues 3–4, November 1980, Pages 271-282, ISSN 0378-1119, http://dx.doi.org/10.1016/0378-1119(80)90067-0. (http://www.sciencedirect.com/science/article/pii/0378111980900670)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:72747
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:13 Dec 2016 15:41
Last Modified:11 Nov 2021 05:06

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