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Molecular organization of a Drosophila puff site that responds to ecdysone

Meyerowitz, Elliot M. and Hogness, David S. (1982) Molecular organization of a Drosophila puff site that responds to ecdysone. Cell, 28 (1). pp. 165-176. ISSN 0092-8674. doi:10.1016/0092-8674(82)90386-5.

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The 68C locus in the polytene chromosomes of Drosophila melanogaster salivary glands is puffed during the last half of the third larval instar and harbors the structural gene for sgs3, one of the glue polypeptides synthesized by the glands during this period. This puff regresses in response to the steroid hormone ecdysone. We have isolated a set of overlapping cloned segments that define approximately 50 kb of DNA at the 68C puff locus. Three polysomal poly(A)+ RNAs that are abundant in the salivary glands during the intermolt-puff stage are transcribed from three genes (II, III and IV) that map near the center of the cloned DNA in a 5 kb cluster region. These are the only transcripts from the 50 kb of 68C DNA detectable in these glands, and they are undetectable in other larval tissues at this stage, or in whole animals at other stages of development. Correlative criteria indicate that gene IV, which yields an RNA of 1.1 kb, is the structural gene for sgs3. Genes II and III, which yield RNAs of 0.36 kb and 0.32 kb, respectively, are oppositely oriented so that their promoters are adjacent, suggesting that this pair may form a single regulated unit, a suggestion that is enhanced by the fact that the pair is bounded by an inverted repeat of 0.3 kb elements. The possible, but as yet unidentified, functions of this gene pair are discussed. A 9.2 kb element belonging to a family of transposable elements called roo is inserted adjacent to the 5 kb cluster region in some but not other D. melanogaster strains. This insertion has no obvious effect on the transcription of genes II, III and IV. Although roo elements yield a 9 kb poly(A)+ RNA in embryos, no roo transcripts were detected in intermolt salivary glands, whether they do or do not contain an element at the 68C puff site.

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Meyerowitz, Elliot M.0000-0003-4798-5153
Additional Information:© 1982 Cell Press. Received September 9, 1981; revised November 2, 1981. We thank W. Bender, K. Burtis, G. Guild, D. Kemp, M. Muskavitch, L. Prestidge and M. Wolfner for their experimental assistance and/or helpful discussions during the course of this work, and M. Crosby, E. Davidson, N. Davidson and T. Maniatis for constructive suggestions regarding the manuscript. This work was supported by grants to D. S. H. from the National Institutes of Health and the National Science Foundation. E. M. M. was a fellow of the Jane Coffin Childs Memorial Fund for Medical Research while at Stanford and received support from an NIH Biomedical Research Support Grant to Caltech for a part of the work done there. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked “advertisement” in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
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Jane Coffin Childs Memorial Fund for Medical ResearchUNSPECIFIED
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Official Citation:Elliot M. Meyerowitz, David S. Hogness, Molecular organization of a Drosophila puff site that responds to ecdysone, Cell, Volume 28, Issue 1, 1982, Pages 165-176, ISSN 0092-8674, (
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:72749
Deposited By: George Porter
Deposited On:13 Dec 2016 15:40
Last Modified:11 Nov 2021 05:06

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