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Isolation and characterization of a cDNA clone for the complete protein coding region of the delta-subunit of the mouse acetylcholine receptor

LaPolla, Robert J. and Mayne, Katharine Mixter and Davidson, Norman (1984) Isolation and characterization of a cDNA clone for the complete protein coding region of the delta-subunit of the mouse acetylcholine receptor. Proceedings of the National Academy of Sciences of the United States of America, 81 (24). pp. 7970-7974. ISSN 0027-8424. PMCID PMC392275. doi:10.1073/pnas.81.24.7970.

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A mouse cDNA clone has been isolated that contains the complete coding region of a protein highly homologous to the δ subunit of the Torpedo acetylcholine receptor (AcChoR). The cDNA library was constructed in the vector λgt10 from membrane-associated poly(A)+ RNA from BC3H-1 mouse cells. Surprisingly, the δ clone was selected by hybridization with cDNA encoding the γ subunit of the Torpedo AcChoR. The nucleotide sequence of the mouse cDNA clone contains an open reading frame of 520 amino acids. This amino acid sequence exhibits 59% and 50% sequence homology to the Torpedo AcChoR δ and γ subunits, respectively. However, the mouse nucleotide sequence has several stretches of high homology with the Torpedo γ subunit cDNA, but not with δ. The mouse protein has the same general structural features as do the Torpedo subunits. It is encoded by a 3.3-kilobase mRNA. There is probably only one, but at most two, chromosomal genes coding for this or closely related sequences.

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Additional Information:© 1984 by the National Academy of Sciences. Contributed by Norman Davidson, August 20, 1984. We are grateful to Dr. Dan Noonan for much help and advice and for providing a Torpedo α cDNA clone, to Dr. Toni Claidio for Torpedo β and δ clones, and to Dr. Steve Heinemann for the Torpedo γ clone (5). Dr. J.P. Merlie provided the BC3H-1 cells. Dr. Barbara Wold provided λgt10 DNA; she and Dr. Charles Rice contributed helpful advice about the cDNA cloning procedure. Tim Hunkapiller provided valuable assistance in the computer analysis of the data. This research has been supported by research grants to N.D. from the National Institutes of Health and the Muscular Dystrophy Association, by a National Institutes of Health Fellowship to R.L.P., and by a National Institutes of Health Predoctoral Traineeship to K.M.M. The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.
Funding AgencyGrant Number
NIH Predoctoral FellowshipUNSPECIFIED
NIH Postdoctoral FellowshipUNSPECIFIED
Muscular Dystrophy AssociationUNSPECIFIED
Subject Keywords:bacteriophage λgt10; sequence homology profiles; BC3H-1 cells
Issue or Number:24
PubMed Central ID:PMC392275
Record Number:CaltechAUTHORS:LAPpnas84
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:7275
Deposited By: Tony Diaz
Deposited On:25 Jan 2007
Last Modified:08 Nov 2021 20:40

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