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Lethal mutations flanking the 68C glue gene cluster on chromosome 3 of Drosophila melanogaster

Cosrby, Madeline A. and Meyerowitz, Elliot M. (1986) Lethal mutations flanking the 68C glue gene cluster on chromosome 3 of Drosophila melanogaster. Genetics, 112 (4). pp. 785-802. ISSN 0016-6731. PMCID PMC1202777.

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We have conducted a genetic analysis of the region flanking the 68C glue gene cluster in Drosophila melanogaster by isolating lethal and semilethal mutations uncovered by deficiencies which span this region. Three different mutagens were used: ethyl methanesulfonate (EMS), ethyl nitrosourea (ENU) and diepoxybutane (DEB). In the region from 68A3 to 68C11, 64 lethal, semilethal, and visible mutations were recovered. These include alleles of 13 new lethal complementation groups, as well as new alleles of rotated, low xanthine dehydrogenase, lethal(3)517 and lethal(3)B76. Six new visible mutations from within this region were recovered on the basis of their reduced viability; all proved to be semiviable alleles of lethal complementation groups. No significant differences were observed in the distributions of lethals recovered using the three different mutagens. Each lethal was mapped on the basis of complementation with overlapping deficiencies; mutations that mapped within the same interval were tested for complementation, and the relative order of the lethal groups within each interval was determined by recombination. The cytological distribution of genes within the 68A3-68C11 region is not uniform: the region from 68A2,3 to 68B1,3 (seven to ten polytene chromosome bands) contains at least 13 lethal complementation groups and the mutation low xanthine dehydrogenase; the adjoining region from 68B1,3 to 68C5,6 (six to nine bands) includes the 68C glue gene cluster, but no known lethal or visible complementation groups; and the interval from 68C5,6 to 68C10,11 (three to five bands) contains at least three lethal complementation groups and the visible mutation rotated. The developmental stage at which lethality is observed was determined for a representative allele from each lethal complementation group.

Item Type:Article
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URLURL TypeDescription CentralArticle
Meyerowitz, Elliot M.0000-0003-4798-5153
Additional Information:© 1986 Genetics Society of America. Manuscript received September 11, 1985. Revised copy accepted November 20, 1985. For expert technical assistance, we wish to thank Elizabeth Noell, Anne Villeneuve and Candice McCoy. This work was supported by grant GM28075 awarded to E.M.M. by the Institute of General Medical Sciences, National Institutes of Health, M.A.C. was supported by a National Research Service Award (T32-GM07616-04) from the Institute of General Medical Sciences, National Institutes of Health. Communicating editor: V. G. FINNERTY
Funding AgencyGrant Number
NIH Predoctoral FellowshipT32-GM07616-04
Issue or Number:4
PubMed Central ID:PMC1202777
Record Number:CaltechAUTHORS:20161213-101916048
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Official Citation:LETHAL MUTATIONS FLANKING THE 68C GLUE GENE CLUSTER ON CHROMOSOME 3 OF DROSOPHILA MELANOGASTER Madeline A. Crosby, Elliot M. Meyerowitz GENETICS April 1, 1986 vol. 112 no. 4 785-802
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:72764
Deposited By: George Porter
Deposited On:13 Dec 2016 20:35
Last Modified:03 Oct 2019 16:21

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