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Sequences sufficient for correct regulation of Sgs-3 lie close to or within the gene

Vijay Raghavan, K. and Crosby, Madeline A. and Mathers, Peter H. and Meyerowitz, Elliot M. (1986) Sequences sufficient for correct regulation of Sgs-3 lie close to or within the gene. EMBO Journal, 5 (12). pp. 3321-3326. ISSN 0261-4189. PMCID PMC1167329. http://resolver.caltech.edu/CaltechAUTHORS:20161213-101916232

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Abstract

The Drosophila melanogaster 68C chromosomal locus is the site of a prominent polytene chromosome puff that harbors the genes Sgs-3, Sgs-7 and Sgs-8. These genes code for proteins that are part of the salivary glue that Drosophila larvae secrete as a means of fixing themselves to an external substrate for the duration of the pre-pupal and pupal period. The 68C glue genes are regulated by the steroid hormone ecdysterone, with the hormone required for both initiation and cessation of gene expression during the third larval instar. Previous work has defined sequences sufficient for expression of abundant levels of Sgs-3 mRNA at the correct time and in the correct tissue. We show here that sequences sufficient for normal tissue- and stage-specific accumulation of Sgs-3 RNA, but adequate only for low levels of expression, lie within 130 bp of the 5' end of the gene, or within the gene.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1167329/PubMed CentralArticle
ORCID:
AuthorORCID
Meyerowitz, Elliot M.0000-0003-4798-5153
Additional Information:© 1986 EMBO. Received on 2 September 1986. Communicated by F. C. Kafatos. We thank Liz Noell, Rita Mundy and Carol Mayeda for expert technical assistance and Mark Garfinkel for pGX2.4 DNA. Helpful comments from Karl Fryxell, Mark Garfinkel, Chris Martin and Margo Roark are acknowledged. This work was supported by grant GM28075 from the National Institutes of Health to E.M.M. M.A.C. and P.H.M. were supported by NIH grant 1 T32 GM07616-08 and K.V.R. was the recipient of a fellowship from Procter and Gamble.
Funders:
Funding AgencyGrant Number
NIHGM-28075
NIH Predoctoral Fellowship1 T32 GM07616-08
Procter and GambleUNSPECIFIED
Subject Keywords:Drosophila / glue / salivary glands/ developmental regulation/ gene expression
PubMed Central ID:PMC1167329
Record Number:CaltechAUTHORS:20161213-101916232
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20161213-101916232
Official Citation: AMA Raghavan KV, Crosby MA, Mathers PH, Meyerowitz EM. Sequences sufficient for correct regulation of Sgs-3 lie close to or within the gene. The EMBO Journal. 1986;5(12):3321-3326.
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:72765
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:13 Dec 2016 21:53
Last Modified:13 Dec 2016 21:53

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