Kress, Horst and Meyerowitz, Elliot M. and Davidson, Norman (1985) High resolution mapping of in situ hybridized biotinylated DNA to surface-spread Drosophila polytene chromosomes. Chromosoma, 93 (2). pp. 113-122. ISSN 0009-5915. doi:10.1007/BF00293158. https://resolver.caltech.edu/CaltechAUTHORS:20161213-103357427
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Abstract
We describe a method of mapping genes or transcripts on polytene chromosomes by transmission electron microscopy. We present several applications which illustrate that, in favorable cases, the method has a resolution of ca. 10 kb, and that high resolution mapping of hybridization sites relative to bands and puffs can be achieved. We mapped sites of transcription for poly-(A) RNA and present evidence which shows that these sites are localized in some bands and puffs, but are also found in interbands.
| Item Type: | Article | |||||||||
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| Additional Information: | © 1985 Springer-Verlag. Received: 02 May 1985; Revised: 08 July 1985. | |||||||||
| Issue or Number: | 2 | |||||||||
| DOI: | 10.1007/BF00293158 | |||||||||
| Record Number: | CaltechAUTHORS:20161213-103357427 | |||||||||
| Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20161213-103357427 | |||||||||
| Official Citation: | Kress, H., Meyerowitz, E.M. & Davidson, N. Chromosoma (1985) 93: 113. doi:10.1007/BF00293158 | |||||||||
| Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | |||||||||
| ID Code: | 72769 | |||||||||
| Collection: | CaltechAUTHORS | |||||||||
| Deposited By: | Tony Diaz | |||||||||
| Deposited On: | 13 Dec 2016 18:37 | |||||||||
| Last Modified: | 11 Nov 2021 05:06 |
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