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High resolution mapping of in situ hybridized biotinylated DNA to surface-spread Drosophila polytene chromosomes

Kress, Horst and Meyerowitz, Elliot M. and Davidson, Norman (1985) High resolution mapping of in situ hybridized biotinylated DNA to surface-spread Drosophila polytene chromosomes. Chromosoma, 93 (2). pp. 113-122. ISSN 0009-5915. http://resolver.caltech.edu/CaltechAUTHORS:20161213-103357427

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Abstract

We describe a method of mapping genes or transcripts on polytene chromosomes by transmission electron microscopy. We present several applications which illustrate that, in favorable cases, the method has a resolution of ca. 10 kb, and that high resolution mapping of hybridization sites relative to bands and puffs can be achieved. We mapped sites of transcription for poly-(A) RNA and present evidence which shows that these sites are localized in some bands and puffs, but are also found in interbands.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1007/BF00293158DOIArticle
http://link.springer.com/article/10.1007%2FBF00293158PublisherArticle
ORCID:
AuthorORCID
Meyerowitz, Elliot M.0000-0003-4798-5153
Additional Information:© 1985 Springer-Verlag. Received: 02 May 1985; Revised: 08 July 1985.
Record Number:CaltechAUTHORS:20161213-103357427
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20161213-103357427
Official Citation:Kress, H., Meyerowitz, E.M. & Davidson, N. Chromosoma (1985) 93: 113. doi:10.1007/BF00293158
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:72769
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:13 Dec 2016 18:37
Last Modified:13 Dec 2016 18:37

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