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Drosophila glue gene Sgs-3: Sequences required for puffing and transcriptional regulation

Crosby, Madeline A. and Meyerowitz, Elliot M. (1986) Drosophila glue gene Sgs-3: Sequences required for puffing and transcriptional regulation. Developmental Biology, 118 (2). pp. 593-607. ISSN 0012-1606. http://resolver.caltech.edu/CaltechAUTHORS:20161213-124141414

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Abstract

The 68C intermolt puff of Drosophila melanogaster contains a cluster of three glue protein genes, Sgs-3, Sgs-7, and Sgs-8. By analysis of chromosomal rearrangements which break near the glue gene cluster, we have established that a region of no more than 20 kb is required for normal expression of the glue genes and for formation of the 68C puff. Using P element-mediated transformation, we have introduced defined segments of the 68C region into the fly genome and assayed the expression of the Sgs-3 gene. Based on the criteria of correct tissue- and stage-specific expression, transcription of an RNA of appropriate size and abundance, and production of an sgs-3 protein, the correctly regulated expression of the Sgs-3 gene requires less than 3.4 kb of total flanking sequences, approximately 2.3 kb 5′ and 1.1 kb 3′. Formation of a new intermolt puff at the site of insertion is not observed for all transformants which produce high levels of Sgs-3 RNA. Only transformants in which the introduced DNA from 68C also contains the Sgs-7 and Sgs-8 genes cause a new intermolt puff at the chromosomal location of the insert.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/0012-1606(86)90029-1DOIArticle
http://www.sciencedirect.com/science/article/pii/0012160686900291PublisherArticle
ORCID:
AuthorORCID
Meyerowitz, Elliot M.0000-0003-4798-5153
Additional Information:© 1986 Elsevier. Received 18 April 1986, Accepted 21 July 1986. We thank Elizabeth Noell for expert technical assistance, and Eric Davidson and members of the Meyerowitz group for critical readings of the manuscript. We thank David Goldberg, James Posakony, Allan Spradling, and Gerald Rubin for generous distribution of P element vectors prior to publication. This work was supported by grant GM28075 awarded to E.M.M. by the Institute of General Medical Sciences, National Institutes of Health. M.A.C. was supported by a National Research Service Award (T32GM07616-04) from the Institute of General Medical Sciences, National Institutes of Health.
Funders:
Funding AgencyGrant Number
NIHGM-28075
NIH Predoctoral FellowshipT32GM07616-04
Record Number:CaltechAUTHORS:20161213-124141414
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20161213-124141414
Official Citation:Madeline A. Crosby, Elliot M. Meyerowitz, Drosophila glue gene Sgs-3: Sequences required for puffing and transcriptional regulation, Developmental Biology, Volume 118, Issue 2, 1986, Pages 593-607, ISSN 0012-1606, http://dx.doi.org/10.1016/0012-1606(86)90029-1. (http://www.sciencedirect.com/science/article/pii/0012160686900291)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:72777
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:13 Dec 2016 22:08
Last Modified:13 Dec 2016 22:08

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