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Evolution and expression of the Sgs-3 glue gene of Drosophila

Martin, Christopher H. and Mayeda, Carol A. and Meyerowitz, Elliot M. (1988) Evolution and expression of the Sgs-3 glue gene of Drosophila. Journal of Molecular Biology, 201 (2). pp. 273-287. ISSN 0022-2836. http://resolver.caltech.edu/CaltechAUTHORS:20161213-142032987

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Abstract

A cluster of three glue genes is present at chromosomal site 68C in the Drosophila melanogaster genome. In this study, we have used a comparative approach to investigate both the regulation and the evolution of the largest of these three genes, Sgs-3. The homologous genes from two related Drosophila species (D. erecta and D. yakuba) have been introduced into the D. melanogaster genome by P-factor-mediated transformation. When the resulting transformant lines were assayed for expression of the introduced genes, near-normal patterns of expression were seen. This demonstrates that the cis-acting regulatory sequences of the introduced Sgs-3-homologous glue genes are capable of interacting effectively with the transcriptional machinery of D. melanogaster. We have also determined the sequences of the Sgs-3-homologous glue genes from D. simulans, D. erecta and D. yakuba. These sequences were compared and used in two ways. The first was to locate conserved sequence elements in regions known to be involved in regulation of the gene. Several such elements were found; they represent potential sites of cis-acting regulatory sequences. Second, we looked at the evolution of the glue gene protein-coding regions. A very rapidly evolving central region of the protein-coding sequences was found; this region contains a striking series of tandem repeats of a five amino acid residue sequence in all four species. Also, a number of conserved aspects of the sgs-3-homologous proteins were found; these features may be essential to their function as a glue.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/0022-2836(88)90138-6DOIArticle
http://www.sciencedirect.com/science/article/pii/0022283688901386PublisherArticle
ORCID:
AuthorORCID
Meyerowitz, Elliot M.0000-0003-4798-5153
Additional Information:© 1988 Elsevier. Received 25 August 1987, Revised 12 January 1988. Wr thank Robert Pruitt, Mark Garfinkel, Charles Rice and Joan Kobori for discussions of sequencing techniques. We also thank the members of the Meyerowitz lab for their helpful suggestions on the manuscript. This work was supported by grant GM28075 from the National Institutes of Health. C.H.M. was supported by a National Science Foundation Fellowship and by a Graduate Fellowship from the General Electric Foundation.
Funders:
Funding AgencyGrant Number
NIHGM-28075
NSF Graduate Research FellowshipUNSPECIFIED
General Electric FoundationUNSPECIFIED
Record Number:CaltechAUTHORS:20161213-142032987
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20161213-142032987
Official Citation:Christopher H. Martin, Carol A. Mayeda, Elliot M. Meyerowitz, Evolution and expression of the Sgs-3 glue gene of Drosophila, Journal of Molecular Biology, Volume 201, Issue 2, 1988, Pages 273-287, ISSN 0022-2836, http://dx.doi.org/10.1016/0022-2836(88)90138-6. (http://www.sciencedirect.com/science/article/pii/0022283688901386)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:72780
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:13 Dec 2016 22:36
Last Modified:13 Dec 2016 22:36

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