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An opsin gene that is expressed only in the R7 photoreceptor cell of Drosophila

Fryxell, Karl J. and Meyerowitz, Elliot M. (1987) An opsin gene that is expressed only in the R7 photoreceptor cell of Drosophila. EMBO Journal, 6 (2). pp. 443-451. ISSN 0261-4189. PMCID PMC553415.

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We have used two techniques to isolate and characterize eye-specific genes from Drosophila melanogaster. First, we identified genes whose expression is limited to eyes, photoreceptor cells, or R7 photoreceptor cells by differential screening with [32P]cDNAs derived from the heads of mutant flies that have reduced amounts of these tissues and cells (Microcephalus, glass3, and sevenless, respectively). Secondly, we identified opsin genes by hybridization with synthetic [32P]oligonucleotides that encode domains that have been conserved between some opsin genes. We found seven clones that contain genes expressed only in the eye or optic lobes of Drosophila; three are expressed only in photoreceptor cells. One is expressed only in R7 photoreceptor cells and hybridizes to some of the previously mentioned oligonucleotides. The complete DNA sequence of the R7-specific opsin gene and its 5' and 3' flanking regions was determined. It is quite different from other known Drosophila opsin genes, in that it is not interrupted by introns and shares only 37-38% amino acid identity with the proteins encoded by these genes. The predicted protein structure contains many characteristics that are common to all rhodopsins, and the sequence differences help to identify four domains of the rhodopsin molecule that have been conserved in evolution.

Item Type:Article
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URLURL TypeDescription CentralArticle
Meyerowitz, Elliot M.0000-0003-4798-5153
Additional Information:© 1987 EMBO. Received on 13 November 1986. Communicated by M. Ashburner. We thank J. Manning for λcDmSO6-559; R. Baker, C. Chang and P. Pang for technical suggestions; C. Martin and R. Pruitt for computer programs; S. Scherer for T4 DNA ligase; R. Mundy for technical assistance; S. Horvath for synthetic oligonucleotides; and our colleagues for comments on the manuscript. K.J.F. is a fellow of the Helen Hay Whitney Foundation. This work was supported by Program Project Grant GM20927 from the NIH. Note added in proof: These sequence data have been submitted to the EMBL/GenBank Data Libraries under the accession number Y00043.
Funding AgencyGrant Number
Helen Hay Whitney FoundationUNSPECIFIED
Subject Keywords:Drosophila; eye; photoreceptor; opsin; rhodopsin
Issue or Number:2
PubMed Central ID:PMC553415
Record Number:CaltechAUTHORS:20161213-142656912
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Official Citation:Fryxell KJ, Meyerowitz EM. An opsin gene that is expressed only in the R7 photoreceptor cell of Drosophila. The EMBO Journal. 1987;6(2):443-451.
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:72781
Deposited By: George Porter
Deposited On:13 Dec 2016 22:41
Last Modified:03 Oct 2019 16:21

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