Williams, Robert W. and Clark, Steven E. and Meyerowitz, Elliot M. (1999) Genetic and physical characterization of a region of Arabidopsis chromosome 1 containing the CLAVATA1 gene. Plant Molecular Biology, 39 (1). pp. 171-176. ISSN 0167-4412. doi:10.1023/A:1006127302671. https://resolver.caltech.edu/CaltechAUTHORS:20161213-144434366
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Abstract
With the advance of Arabidopsis as a model system for understanding plant genetics, development and biochemistry, a detailed description of the genome is necessary. As such, focused projects are underway to map and sequence the Arabidopsis nuclear genome. We have characterized a region of chromosome 1, surrounding the CLAVATA1 (CLV1) locus. Three (RFLP) clones were mapped relative to clv1-1, and were used to construct an ca. 700 kb yeast artificial chromosome (YAC) contig. Three cosmids spanning the CLV1 locus were analyzed and ca. 24 kb of genomic DNA was sequenced, including a continuous stretch of 18 kb. In addition to generating clones in this region of chromosome 1, we have analyzed the size, spacing and organization of several contiguous genes.
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| Additional Information: | © 1999 Kluwer Academic Publishers. Received 24 March 1998; accepted in revised form 6 July 1998. We thank Hajime Sakai and Leonard Medrano for technical advice. We also acknowledge Xuemei Chen, Jennifer Fletcher, Toshiro Ito, Prakash Kumar, Jose-Luis Riechmann, Kevin Roberg, Mark Running and Eva Ziegelhoffer for careful review of this manuscript. ga2 seeds were provided by the Arabidopsis Biological Resource Center at The Ohio State University. This work was supported by NSF grant MCB-9204839 and a Strategic Research Fund Grant from Zeneca Seeds to E.M.M. S.E.C. was a NSF Plant Biology Postdoctoral Fellow and R.W.W. was supported by NIH Predoctoral Training Grant GM07616 and the Howard Hughes Medical Institute. The nucleotide sequence data reported will appear in the EMBL, GenBank and DDBJ Nucleotide Sequence Databases under the accession number AF049870. | ||||||||||||
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| Subject Keywords: | Arabidopsis, CLAVATA1, genome sequencing, RFLP, YAC contig | ||||||||||||
| Issue or Number: | 1 | ||||||||||||
| DOI: | 10.1023/A:1006127302671 | ||||||||||||
| Record Number: | CaltechAUTHORS:20161213-144434366 | ||||||||||||
| Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20161213-144434366 | ||||||||||||
| Official Citation: | Williams, R.W., Clark, S.E. & Meyerowitz, E.M. Plant Mol Biol (1999) 39: 171. doi:10.1023/A:1006127302671 | ||||||||||||
| Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | ||||||||||||
| ID Code: | 72784 | ||||||||||||
| Collection: | CaltechAUTHORS | ||||||||||||
| Deposited By: | George Porter | ||||||||||||
| Deposited On: | 14 Dec 2016 17:29 | ||||||||||||
| Last Modified: | 11 Nov 2021 05:06 |
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