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Cooperative enhancement at the Drosophila Sgs-3 locus

Roark, Margaret and Vijay Raghavan, K. and Todo, Takeshi and Mayeda, Carol A. and Meyerowitz, Elliot M. (1990) Cooperative enhancement at the Drosophila Sgs-3 locus. Developmental Biology, 139 (1). pp. 121-133. ISSN 0012-1606. doi:10.1016/0012-1606(90)90283-O.

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The Drosophila glue gene Sgs-3 is specifically expressed in the secretory cells of the salivary glands of third instar larvae. We have assayed the expression of gene fusions to determine the role of cis-acting Sgs-3 sequences in conferring this pattern of expression. These experiments define two regulatory regions required for expression of reporter genes from the Sgs-3 promoter. One region, between 106 and 56 bp upstream of the Sgs-3 mRNA 5′ end is sufficient for low but correct tissue- and stage-specific expression. A second region, lying between 629 and 130 bp 5′ of the RNA start site is functionally equivalent; that is, it alone will also direct low level, specific expression. These two regions act synergistically to give high level expression. More distant upstream regions function to further increase levels of expression. These two regulatory elements can confer a salivary gland-specific pattern of expression on a heterologous promoter and are also sufficient to drive gene expression in other Drosophila species, implying conservation of regulators.

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Meyerowitz, Elliot M.0000-0003-4798-5153
Additional Information:© 1990 Elsevier. Accepted 29 November 1989. We are grateful to those, already mentioned in the text, who have generously shared plasmids or unpublished data with us. We are particularly indebted to Sherry A. Kempin for technical help and to B. A. Hamilton, P. H. Mathers, and M. J. Palazzolo for critical reading of the manuscript. Our work is supported by grants from the National Institutes of Health (GM 28075) and the Lucille P. Markey Charitable Trust. M.R. was supported by NIH Postdoctoral Fellowship GM 10694.
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Lucille P. Markey Charitable TrustUNSPECIFIED
NIH Postdoctoral FellowshipGM-10694
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Record Number:CaltechAUTHORS:20161214-114033267
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Official Citation:Margaret Roark, K.Vijay Raghavan, Takeshi Todo, Carol A. Mayeda, Elliot M. Meyerowitz, Cooperative enhancement at the Drosophila Sgs-3 locus, Developmental Biology, Volume 139, Issue 1, 1990, Pages 121-133, ISSN 0012-1606, (
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ID Code:72816
Deposited By: George Porter
Deposited On:14 Dec 2016 21:08
Last Modified:11 Nov 2021 05:07

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