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AGL1-AGL6, an Arabidopsis gene family with similarity to floral homeotic and transcription factor genes

Ma, Hong and Yanofsky, Martin F. and Meyerowitz, Elliot M. (1991) AGL1-AGL6, an Arabidopsis gene family with similarity to floral homeotic and transcription factor genes. Genes and Development, 5 (3). pp. 484-495. ISSN 0890-9369. https://resolver.caltech.edu/CaltechAUTHORS:20161214-115507463

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Abstract

The predicted products of floral homeotic genes, AGAMOUS (AG) from Arabidopsis thaliana and DEFICIENS A (DEF A) from Antirrhinum majus, have been shown previously to share strong sequence similarity with transcription factors from humans (SRF) and yeast (MCM1). The conserved sequence between these proteins is localized within a domain known to be necessary for the DNA binding and for the dimerization of SRF. We have isolated six new genes from A. thaliana, AGL1-AGL6, which also have this conserved sequence motif. On the basis of the sequence comparison between the AG and AGL genes, they can be assigned to two subfamilies of a large gene family. RNA dot blot analysis indicates that five of these genes (AGL1, AGL2, AGL4, AGL5, and AGL6) are preferentially expressed in flowers. In addition, in situ RNA hybridization experiments with AGL1 and AGL2 show that their mRNAs are detected in some floral organs but not in others. Our results suggest that these genes may act to control many steps of Arabidopsis floral morphogenesis. In contrast, the AGL3 gene is expressed in vegetative tissues as well as in flowers, suggesting that it functions in a broader range of tissues. We discuss possible roles of this gene family during the evolution of flowers.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1101/gad.5.3.484DOIArticle
http://genesdev.cshlp.org/content/5/3/484.abstractPublisherArticle
ORCID:
AuthorORCID
Meyerowitz, Elliot M.0000-0003-4798-5153
Additional Information:© 1991 Cold Spring Harbor Laboratory Press. The Authors acknowledge that six months after the full-issue publication date, the Article will be distributed under a Creative Commons CC-BY-NC License (Attribution-NonCommercial 4.0 International License, http://creativecommons.org/licenses/by-nc/4.0/). Received October 30, 1990; revised version accepted December 28, 1990. We thank S. Kempin for help with RFLP mapping, L. Medrano for excellent technical assistance, G. Drews and I. Bowman for advice on the in situ hybridization experiments, D. Spector and L. Fields for help with photography of in situ slides, A. Tagle for assistance in preparation of this manuscript, and I. Bowman, L. Brockman, C. Chang, G. Drews, B. Hamilton, T. lack, L. Sieburth, V. Sundaresan, A. van der Bliek, and D.Weigel for valuable comments on this manuscript. This work was supported by grants from the National Science Foundation (DCB 8703439) and the Lucille P. Markey Charitable Trust. H.M. was supported by a postdoctoral fellowship from the Helen Hay Whitney Foundation, and M.F.Y. was supported by a postdoctoral fellowship in plant biology from the National Science Foundation {DCB 8608456).The sequence data described in this paper have been submitted to the GenBank database, and the accession numbers are AGL1, M55550; AGL2, M55551; AGL4, M55552; AGLS, M55553; and AGL6, M55554. The publication costs of this article were defrayed in part by payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 USC section 1734 solely to indicate this fact.
Funders:
Funding AgencyGrant Number
NSFDCB-8703439
Lucille P. Markey Charitable TrustUNSPECIFIED
Helen Hay Whitney FoundationUNSPECIFIED
NSF Postdoctoral FellowshipDCB-8608456
Subject Keywords:Floral-specific genes; flower development; gene family; MADS box; in situ hybridization
Issue or Number:3
Record Number:CaltechAUTHORS:20161214-115507463
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20161214-115507463
Official Citation:AGL1-AGL6, an Arabidopsis gene family with similarity to floral homeotic and transcription factor genes. H Ma, M F Yanofsky, and E M Meyerowitz Genes Dev. March 1991 5: 484-495; doi:10.1101/gad.5.3.484
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:72818
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:14 Dec 2016 21:35
Last Modified:03 Oct 2019 16:21

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