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Rapid isolation of long cDNA clones from existing libraries

Hamilton, Bruce A. and Palazzolo, Michael J. and Meyerowitz, Elliot M. (1991) Rapid isolation of long cDNA clones from existing libraries. Nucleic Acids Research, 19 (8). pp. 1951-1952. ISSN 0305-1048. PMCID PMC328137. https://resolver.caltech.edu/CaltechAUTHORS:20161214-120431504

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Abstract

Obtaining full-length or even near full-length cDNA clones has been a time-consuming and labor-intensive step in the analysis of cloned genes. Recent methods which use PCR as a preparative tool for cloning (1,2) have made this step considerably more rapid, but may introduce sequence errors in the resulting clones due to numerous sequential rounds of in vitro replication. We describe a method for identifying long cDNA clones from existing cDNA libraries using PCR purely as a diagnostic tool.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1093/nar/19.8.1951DOIArticle
http://nar.oxfordjournals.org/content/19/8/1951.full.pdf+htmlPublisherArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC328137/PubMed CentralArticle
ORCID:
AuthorORCID
Meyerowitz, Elliot M.0000-0003-4798-5153
Additional Information:© 1991 Oxford University Press. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. Submitted February 5, 1991. We thank M. Strathmann, K.Vijay Raghavan, and T.Wilke for constructive discussions and C.Mayeda and M.Whitney for excellent technical assistance. B.A.H. was supported in part by a USPHS Predoctoral National Research Service Award (T32 GM07616). This work was supported by a scholar's grant from the Lucille P.Markey Charitable Trust (M.J.P.) and USPHS Program Project Grant GM40499 (E.M.M.).
Funders:
Funding AgencyGrant Number
NIH Predoctoral FellowshipT32 GM07616
Lucille P. Markey Charitable TrustUNSPECIFIED
NIHGM40499
Issue or Number:8
PubMed Central ID:PMC328137
Record Number:CaltechAUTHORS:20161214-120431504
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20161214-120431504
Official Citation:Bruce A. Hamilton, Michael J. Palazzolo, and Elliot M. Meyerowitz Rapid isolation of long cDNA clones from existing libraries Nucl. Acids Res. (1991) 19 (8): 1951-1952 doi:10.1093/nar/19.8.1951
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:72819
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:14 Dec 2016 21:37
Last Modified:03 Oct 2019 16:21

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