Rapid isolation of long cDNA clones from existing libraries

Hamilton, Bruce A. and Palazzolo, Michael J. and Meyerowitz, Elliot M. (1991) Rapid isolation of long cDNA clones from existing libraries. Nucleic Acids Research, 19 (8). pp. 1951-1952. ISSN 0305-1048. PMCID PMC328137. doi:10.1093/nar/19.8.1951. https://resolver.caltech.edu/CaltechAUTHORS:20161214-120431504

PDF - Published Version
Creative Commons Attribution Non-commercial.
331kB

Use this Persistent URL to link to this item: https://resolver.caltech.edu/CaltechAUTHORS:20161214-120431504

Abstract

Obtaining full-length or even near full-length cDNA clones has been a time-consuming and labor-intensive step in the analysis of cloned genes. Recent methods which use PCR as a preparative tool for cloning (1,2) have made this step considerably more rapid, but may introduce sequence errors in the resulting clones due to numerous sequential rounds of in vitro replication. We describe a method for identifying long cDNA clones from existing cDNA libraries using PCR purely as a diagnostic tool.

Item Type:

Article

Related URLs:

URL	URL Type	Description
http://dx.doi.org/10.1093/nar/19.8.1951	DOI	Article
http://nar.oxfordjournals.org/content/19/8/1951.full.pdf+html	Publisher	Article
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC328137/	PubMed Central	Article

ORCID:

Author	ORCID
Meyerowitz, Elliot M.	0000-0003-4798-5153

Additional Information:

© 1991 Oxford University Press. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. Submitted February 5, 1991. We thank M. Strathmann, K.Vijay Raghavan, and T.Wilke for constructive discussions and C.Mayeda and M.Whitney for excellent technical assistance. B.A.H. was supported in part by a USPHS Predoctoral National Research Service Award (T32 GM07616). This work was supported by a scholar's grant from the Lucille P.Markey Charitable Trust (M.J.P.) and USPHS Program Project Grant GM40499 (E.M.M.).

Funders:

Funding Agency	Grant Number
NIH Predoctoral Fellowship	T32 GM07616
Lucille P. Markey Charitable Trust	UNSPECIFIED
NIH	GM40499

Issue or Number:

PubMed Central ID:

PMC328137

DOI:

10.1093/nar/19.8.1951

Record Number:

CaltechAUTHORS:20161214-120431504

Persistent URL:

https://resolver.caltech.edu/CaltechAUTHORS:20161214-120431504

Official Citation:

Bruce A. Hamilton, Michael J. Palazzolo, and Elliot M. Meyerowitz Rapid isolation of long cDNA clones from existing libraries Nucl. Acids Res. (1991) 19 (8): 1951-1952 doi:10.1093/nar/19.8.1951

Usage Policy:

No commercial reproduction, distribution, display or performance rights in this work are provided.

ID Code:

72819

Collection:

CaltechAUTHORS

Deposited By:

George Porter

Deposited On:

14 Dec 2016 21:37

Last Modified:

11 Nov 2021 05:07

Repository Staff Only: item control page