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Temperature-Sensitive Splicing in the Floral Homeotic Mutant apetala3-1

Sablowski, Robert W. M. and Meyerowitz, Elliot M. (1998) Temperature-Sensitive Splicing in the Floral Homeotic Mutant apetala3-1. Plant Cell, 10 (9). pp. 1453-1463. ISSN 1040-4651. PMCID PMC144071. https://resolver.caltech.edu/CaltechAUTHORS:20161214-121625447

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Abstract

The floral homeotic gene APETALA3 (AP3) is required for stamen and petal development in Arabidopsis. The previously described ap3-1 allele is temperature sensitive and carries a missense mutation near a 5′ splice site. The missense mutation lies within a domain of the AP3 protein that is thought to be important for protein–protein interactions, which suggests that temperature sensitivity of ap3-1 could reflect an unstable interaction with cofactors. Here, we show instead that the ap3-1 mutation causes a temperature-dependent splicing defect and that temperature sensitivity is not a property of the protein products of ap3-1 but of RNA processing, possibly because of unstable base pairing between the transcript and small nuclear RNAs. The unexpected defect of the ap3-1 mutant offers unique opportunities for genetic and molecular studies of splice site recognition in plants.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1105/tpc.10.9.1453DOIArticle
http://www.plantcell.org/content/10/9/1453.longPublisherArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC144071/PubMed CentralArticle
ORCID:
AuthorORCID
Meyerowitz, Elliot M.0000-0003-4798-5153
Additional Information:© 1998 American Society of Plant Physiologists. Received April 20, 1998; accepted July 13, 1998. Published September 1, 1998. We are grateful to Dr. Tom Jack for the pF733 plasmid and anti-AP3 antiserum and for communicating results before publication and to Drs. Gordon Simpson, Tracy Johnson, John Wagner, Tom Jack, Jennifer Fletcher, Prakash Kumar, Steve Jacobsen, Eva Ziegelhoffer, Carolyn Ohno, and Chiou-Fen Chuang for critical reading of the manuscript. R.W.M.S. was supported by a long-term fellowship (No. LT-12/95) from the Human Frontier Science Program, and work in E.M.M.'s laboratory is supported by a grant from the National Science Foundation (No. MCB-9603821).
Funders:
Funding AgencyGrant Number
Human Frontier Science ProgramLT-12/95
NSFMCB-9603821
Issue or Number:9
PubMed Central ID:PMC144071
Record Number:CaltechAUTHORS:20161214-121625447
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20161214-121625447
Official Citation:Sablowski RW, Meyerowitz EM. Temperature-sensitive splicing in the floral homeotic mutant apetala3-1. The Plant Cell. 1998;10(9):1453-1463.
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:72820
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:14 Dec 2016 21:49
Last Modified:03 Oct 2019 16:21

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