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Distinct forms of the ß subunit of GTP-binding regulatory proteins identified by molecular cloning

Fong, Henry K. W. and Amatruda, Thomas T., III and Birren, Bruce W. and Simon, Melvin I. (1987) Distinct forms of the ß subunit of GTP-binding regulatory proteins identified by molecular cloning. Proceedings of the National Academy of Sciences of the United States of America, 84 (11). pp. 3792-3796. ISSN 0027-8424. PMCID PMC304962. doi:10.1073/pnas.84.11.3792.

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Two distinct β subunits of guanine nucleotide-binding regulatory proteins have been identified by cDNA cloning and are referred to as β 1 and β 2 subunits. The bovine transducin β subunit (β 1) has been cloned previously. We have now isolated and analyzed cDNA clones that encode the β 2 subunit from bovine adrenal, bovine brain, and a human myeloid leukemia cell line, HL-60. The 340-residue Mr 37,329 β 2 protein is 90% identical with β 1 in predicted amino acid sequence, and it is also organized as a series of repetitive homologous segments. The major mRNA that encodes the bovine β 2 subunit is 1.7 kilobases in length. It is expreβed at lower levels than β 1 subunit mRNA in all tiβues examined. The β 1 and β 2 meβages are expreβed in cloned human cell lines. Hybridization of cDNA probes to bovine DNA showed that β 1 and β 2 are encoded by separate genes. The amino acid sequences for the bovine and human β 2 subunit are identical, as are the amino acid sequences for the bovine and human β 1 subunit. This evolutionary conservation suggests that the two β subunits have different roles in the signal transduction process.

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Additional Information:© 1987 by the National Academy of Sciences. Contributed by Melvin I. Simon, February 27, 1987. We express sincere thanks to Dr. Barbara Wold and Dr. Ryn Miake-Lye for helpful discussions and for providing us with the bovine adrenal and brain cDNA libraries, to Cathy H. W. Chang and Kenneth K. Yoshimoto for technical assistance, and to Cathy Elkins for help in preparation of the manuscript. This work was supported by a Physician's Research Training fellowship from the American Cancer Society (to T.T.A.), National Institutes of Health Postdoctoral Fellowship Award GM10974 (to B.W.B.), and Grant GM34236 from the National Institutes of Health. The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.
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American Cancer SocietyUNSPECIFIED
NIH Predoctoral FellowshipGM10974
Subject Keywords:signal transduction; molecular evolution; human myeloid HL-60 cells
Issue or Number:11
PubMed Central ID:PMC304962
Record Number:CaltechAUTHORS:FONpnas87
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:7285
Deposited By: Tony Diaz
Deposited On:26 Jan 2007
Last Modified:08 Nov 2021 20:41

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