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Confocal Microscopy of the Shoot Apex

Running, Mark P. and Clark, Steven E. and Meyerowitz, Elliot M. (1995) Confocal Microscopy of the Shoot Apex. In: Methods in Cell Biology. Vol.49. Elsevier , New York, NY, pp. 217-229. ISBN 978-0-12-564151-7.

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This chapter focuses on confocal laser scanning microscopy (CLSM). In CLSM, a sample is treated with a fluorescent dye, and a laser beam is focused on a point in the sample, causing the emission of fluorescent light. The use of CLSM in the study of plant development has many advantages over conventional histological techniques. Because CLSM data are stored digitally, the data can not only be reconstructed in three dimensions, but also animated or displayed as stereo images, to aid in visual interpretation. Once the sample is reconstructed on a computer, virtual sections can be made from any orientation. Specimen preparation for CLSM is also much less labor intensive compared to serial sectioning, and avoids problems such as recovering and mounting individual sections, a time-consuming and error-prone task. The integrity of the sample is preserved in CLSM, allowing rescanning using different parameters. The chapter describes the method of examining plant tissue using CLSM, particularly in studies of the structure, cell number, and cell pattern of shoot apical meristems and floral meristems. The development of this technique is timely because of the recent explosion of interest in developmental mutants that affect apical and floral meristem structure.

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Meyerowitz, Elliot M.0000-0003-4798-5153
Additional Information:© 1995 Academic Press. We thank C. Chang, S. Jacobsen, Z. Liu, J.-L. Riechmann, H. Sakai, L. Sieberth, and B. Williams for review of the manuscript, and A. Readhead for help with tissue preparation. We especially thank Dr. Jean-Paul Revel for advice and help with using the CLSM instrument, advice and suggestions for sample preparation, and review of the manuscript. This work was supported by U.S. National Science Foundation (NSF) Grant MCB-9204839 to E.M.M. M.P.R. was a Howard Hughes Predoctoral Fellow, and S.E.C. was supported by an NSF Postdoctoral Fellowship in Plant Biology.
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Howard Hughes Medical Institute (HHMI)UNSPECIFIED
NSF Plant Biology Postdoctoral FellowshipUNSPECIFIED
Record Number:CaltechAUTHORS:20161215-122419408
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Official Citation:Mark P. Running, Steven E. Clark, Elliot M. Meyerowitz, Chapter 15 Confocal Microscopy of the Shoot Apex, In: David W. Galbraith, Hans J. Bohnert and Don P. Bourque, Editor(s), Methods in Cell Biology, Academic Press, 1995, Volume 49, Pages 217-229, ISSN 0091-679X, ISBN 9780125641517, (
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:72852
Deposited By: George Porter
Deposited On:15 Dec 2016 21:51
Last Modified:11 Nov 2021 05:07

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