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Genetic separation of third and fourth whorl functions of AGAMOUS

Sieburth, Leslie E. and Running, Mark P. and Meyerowitz, Elliot M. (1995) Genetic separation of third and fourth whorl functions of AGAMOUS. Plant Cell, 7 (8). pp. 1249-1258. ISSN 1040-4651. PMCID PMC160948. https://resolver.caltech.edu/CaltechAUTHORS:20161216-093109349

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Abstract

AGAMOUS (AG) is an Arabidopsis MADS box gene required for normal development of the third and fourth whorls of the flower. In previously described ag mutants, the third whorl stamens are replaced by petals, and the fourth whorl is replaced by another (mutant) flower. We describe two new ag alleles, ag-4 and AG-Met205, retaining partial AG activity. Both produce flowers with stamens in the third whorl and indeterminate floral meristems; however, ag-4 flowers contain sepals in the fourth whorl, and AG-Met205 produces carpels. The ag-4 mutation results in partial loss of the C terminus of the K domain, a putative coiled coil, and AG-Met205 contains a site-directed mutation that causes a single amino acid change in this same region of the K box. Two models that might explain how these changes in AG result in the separation of different AG activities are discussed.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1105/tpc.7.8.1249DOIArticle
http://www.plantcell.org/content/7/8/1249.abstractPublisherArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC160948/PubMed CentralArticle
ORCID:
AuthorORCID
Meyerowitz, Elliot M.0000-0003-4798-5153
Additional Information:© 1995 American Society of Plant Biologists. Received March 27, 1995; accepted May 10, 1995. We thank David Smyth and John Alvarez for the generous gift of the ag-4 allele and Esther Koh for assistance with the AG-Mef205 transformations. We also thank 60th Krizek, Zhongchi Liu, Jose Luis Riechmann, Caren Chang, Joshua Z. Levin, Hajime Sakai, Steve Jacobsen, Jian Hua, and Justin Goodrich for their comments on the manuscript. M.P.R. was a Howard Hughes Predoctoral Fellow, and L.E.S. was a Damon Runyon-Walter Winchell Cancer Research Fellow (Grant No. DRG1081). This work was supported by National lnstitutes of Health Grant No. GM45697 to E.M.M.
Funders:
Funding AgencyGrant Number
Howard Hughes Medical Institute (HHMI)UNSPECIFIED
Damon Runyon-Walter Winchell Cancer FundDRG1081
NIHGM45697
Issue or Number:8
PubMed Central ID:PMC160948
Record Number:CaltechAUTHORS:20161216-093109349
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20161216-093109349
Official Citation:Sieburth LE, Running MP, Meyerowitz EM. Genetic separation of third and fourth whorl functions of AGAMOUS. The Plant Cell. 1995;7(8):1249-1258. doi:10.1105/tpc.7.8.1249.
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:72890
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:16 Dec 2016 19:15
Last Modified:03 Oct 2019 16:22

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