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Triggered amplification by hybridization chain reaction

Dirks, Robert M. and Pierce, Niles A. (2004) Triggered amplification by hybridization chain reaction. Proceedings of the National Academy of Sciences of the United States of America, 101 (43). pp. 15275-15278. ISSN 0027-8424. PMCID PMC524468.

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We introduce the concept of hybridization chain reaction (HCR), in which stable DNA monomers assemble only upon exposure to a target DNA fragment. In the simplest version of this process, two stable species of DNA hairpins coexist in solution until the introduction of initiator strands triggers a cascade of hybridization events that yields nicked double helices analogous to alternating copolymers. The average molecular weight of the HCR products varies inversely with initiator concentration. Amplification of more diverse recognition events can be achieved by coupling HCR to aptamer triggers. This functionality allows DNA to act as an amplifying transducer for biosensing applications.

Item Type:Article
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URLURL TypeDescription CentralArticle
Pierce, Niles A.0000-0003-2367-4406
Additional Information:© 2004 The National Academy of Sciences of the USA. From the cover. Communicated by Stephen L. Mayo, California Institute of Technology, Pasadena, CA, September 24, 2004 (received for review July 2, 2004). Published online before print October 18, 2004, 10.1073/pnas.0407024101 This work was supported by the Ralph M. Parsons Foundation and the Charles Lee Powell Foundation.
Funding AgencyGrant Number
Ralph M. Parsons FoundationUNSPECIFIED
Charles Lee Powell FoundationUNSPECIFIED
Issue or Number:43
PubMed Central ID:PMC524468
Record Number:CaltechAUTHORS:DIRpnas04
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:730
Deposited By: Archive Administrator
Deposited On:21 Sep 2005
Last Modified:02 Oct 2019 22:36

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