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Quantitative phase imaging and complex field reconstruction by pupil modulation differential phase contrast

Lu, Hangwen and Chung, Jaebum and Ou, Xiaoze and Yang, Changhuei (2016) Quantitative phase imaging and complex field reconstruction by pupil modulation differential phase contrast. Optics Express, 24 (22). pp. 25345-25361. ISSN 1094-4087. https://resolver.caltech.edu/CaltechAUTHORS:20161222-112221157

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Abstract

Differential phase contrast (DPC) is a non-interferometric quantitative phase imaging method achieved by using an asymmetric imaging procedure. We report a pupil modulation differential phase contrast (PMDPC) imaging method by filtering a sample’s Fourier domain with half-circle pupils. A phase gradient image is captured with each half-circle pupil, and a quantitative high resolution phase image is obtained after a deconvolution process with a minimum of two phase gradient images. Here, we introduce PMDPC quantitative phase image reconstruction algorithm and realize it experimentally in a 4f system with an SLM placed at the pupil plane. In our current experimental setup with the numerical aperture of 0.36, we obtain a quantitative phase image with a resolution of 1.73μm after computationally removing system aberrations and refocusing. We also extend the depth of field digitally by 20 times to ±50μm with a resolution of 1.76μm.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1364/OE.24.025345DOIArticle
https://www.osapublishing.org/oe/abstract.cfm?uri=oe-24-22-25345PublisherArticle
ORCID:
AuthorORCID
Chung, Jaebum0000-0003-3932-8428
Yang, Changhuei0000-0001-8791-0354
Additional Information:© 2016 Optical Society of America. Received 26 Jul 2016; revised 15 Oct 2016; accepted 18 Oct 2016; published 21 Oct 2016. The authors would like to thank Daniel Martin for fabricating the Siemens star phase target, and thanks to Jinho Kim, Joshua Brake and Haowen Ruan for discussions and help with experiments. Funding: NIH (grant no. R01 AI096226); CII 2015 (grant no. 32070065).
Funders:
Funding AgencyGrant Number
NIHR01 AI096226
Caltech Innovation Initiative (CI2)32070065
Issue or Number:22
Classification Code:OCIS codes: (100.5070) Phase retrieval; (170.0180) Microscopy; (110.3010) Image reconstruction techniques
Record Number:CaltechAUTHORS:20161222-112221157
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20161222-112221157
Official Citation:Hangwen Lu, Jaebum Chung, Xiaoze Ou, and Changhuei Yang, "Quantitative phase imaging and complex field reconstruction by pupil modulation differential phase contrast," Opt. Express 24, 25345-25361 (2016)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:73152
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:22 Dec 2016 19:55
Last Modified:09 Mar 2020 13:18

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