Lu, Hangwen and Chung, Jaebum and Ou, Xiaoze and Yang, Changhuei (2016) Quantitative phase imaging and complex field reconstruction by pupil modulation differential phase contrast. Optics Express, 24 (22). pp. 25345-25361. ISSN 1094-4087. https://resolver.caltech.edu/CaltechAUTHORS:20161222-112221157
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Abstract
Differential phase contrast (DPC) is a non-interferometric quantitative phase imaging method achieved by using an asymmetric imaging procedure. We report a pupil modulation differential phase contrast (PMDPC) imaging method by filtering a sample’s Fourier domain with half-circle pupils. A phase gradient image is captured with each half-circle pupil, and a quantitative high resolution phase image is obtained after a deconvolution process with a minimum of two phase gradient images. Here, we introduce PMDPC quantitative phase image reconstruction algorithm and realize it experimentally in a 4f system with an SLM placed at the pupil plane. In our current experimental setup with the numerical aperture of 0.36, we obtain a quantitative phase image with a resolution of 1.73μm after computationally removing system aberrations and refocusing. We also extend the depth of field digitally by 20 times to ±50μm with a resolution of 1.76μm.
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| Additional Information: | © 2016 Optical Society of America. Received 26 Jul 2016; revised 15 Oct 2016; accepted 18 Oct 2016; published 21 Oct 2016. The authors would like to thank Daniel Martin for fabricating the Siemens star phase target, and thanks to Jinho Kim, Joshua Brake and Haowen Ruan for discussions and help with experiments. Funding: NIH (grant no. R01 AI096226); CII 2015 (grant no. 32070065). | |||||||||
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| Issue or Number: | 22 | |||||||||
| Classification Code: | OCIS codes: (100.5070) Phase retrieval; (170.0180) Microscopy; (110.3010) Image reconstruction techniques | |||||||||
| Record Number: | CaltechAUTHORS:20161222-112221157 | |||||||||
| Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20161222-112221157 | |||||||||
| Official Citation: | Hangwen Lu, Jaebum Chung, Xiaoze Ou, and Changhuei Yang, "Quantitative phase imaging and complex field reconstruction by pupil modulation differential phase contrast," Opt. Express 24, 25345-25361 (2016) | |||||||||
| Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | |||||||||
| ID Code: | 73152 | |||||||||
| Collection: | CaltechAUTHORS | |||||||||
| Deposited By: | George Porter | |||||||||
| Deposited On: | 22 Dec 2016 19:55 | |||||||||
| Last Modified: | 09 Mar 2020 13:18 |
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