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Compact, lensless digital holographic microscope for remote microbiology

Serabyn, Eugene and Liewer, Kurt and Lindensmith, Chris and Wallace, Kent and Nadeau, Jay (2016) Compact, lensless digital holographic microscope for remote microbiology. Optics Express, 24 (25). pp. 28540-28548. ISSN 1094-4087. http://resolver.caltech.edu/CaltechAUTHORS:20170105-095902664

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Abstract

In situ investigation of microbial life in extreme environments can be carried out with microscopes capable of imaging 3-dimensional volumes and tracking particle motion. Here we present a lensless digital holographic microscope approach that provides roughly 1.5 micron resolution in a compact, robust package suitable for remote deployment. High resolution is achieved by generating high numerical-aperture input beams with radial gradient-index rod lenses. The ability to detect and track prokaryotes was explored using bacterial strains of two different sizes. In the larger strain, a variety of motions were seen, while the smaller strain was used to demonstrate a detection capability down to micron scales.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1364/OE.24.028540DOIArticle
https://www.osapublishing.org/oe/abstract.cfm?uri=oe-24-25-28540PublisherArticle
ORCID:
AuthorORCID
Nadeau, Jay0000-0001-5258-0076
Additional Information:© 2016 Optical Society of America. Received 20 Sep 2016; revised 21 Nov 2016; accepted 21 Nov 2016; published 1 Dec 2016. Part of this work was carried out at the Jet Propulsion Laboratory, California Institute of Technology, under contract with NASA. We thank J. Deming (UW) for providing the Colwellia strain, and S. Rider (Caltech) for assistance with mechanical engineering. Funding: NASA Jet Propulsion Laboratory; Gordon and Betty Moore Foundation (4037, 4038).
Group:GALCIT
Funders:
Funding AgencyGrant Number
NASA/JPL/CaltechUNSPECIFIED
Gordon and Betty Moore Foundation4037
Gordon and Betty Moore Foundation4038
Classification Code:OCIS codes: (090.1995) Digital holography; (100.3175) Interferometric imaging; (120.4570) Optical design of instruments.
Record Number:CaltechAUTHORS:20170105-095902664
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20170105-095902664
Official Citation:Eugene Serabyn, Kurt Liewer, Chris Lindensmith, Kent Wallace, and Jay Nadeau, "Compact, lensless digital holographic microscope for remote microbiology," Opt. Express 24, 28540-28548 (2016)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:73262
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:05 Jan 2017 21:13
Last Modified:31 Oct 2017 18:30

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