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Multimode optical imaging for translational chemotherapy: in vivo tumor detection and delineation by targeted gallium corroles

Hwang, Jae Youn and Gross, Zeev and Gray, Harry B. and Medina-Kauwe, Lali K. and Farkas, Daniel L. (2011) Multimode optical imaging for translational chemotherapy: in vivo tumor detection and delineation by targeted gallium corroles. In: Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IX. Proceedings of SPIE. No.7902. Society of Photo-Optical Instrumentation Engineers (SPIE) , Bellingham, WA, Art. No. 79020F. ISBN 978-0-81948-439-0. PMCID PMC4582672. http://resolver.caltech.edu/CaltechAUTHORS:20170111-103042462

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Abstract

We report the feasibility of tumor detection and delineation in vivo using multimode optical imaging of targeted gallium corrole (HerGa). HerGa is highly effective for targeted HER2+ tumor elimination in vivo, and it emits intense fluorescence. These unique characteristics of HerGa prompted us to investigate the potential of HerGa for tumor detection and delineation, by performing multimode optical imaging ex vivo and in vivo; the imaging modes included fluorescence intensity, spectral (including ratiometric), lifetime, and two-photon excited fluorescence, using our custombuilt imaging system. While fluorescence intensity imaging provided information about tumor targeting capacity and tumor retention of HerGa, ratiometric spectral imaging offered more quantitative and specific information about HerGa location and accumulation. Most importantly, the fluorescence lifetime imaging of HerGa allowed us to discriminate between tumor and non-tumor regions by fluorescence lifetime differences. Finally, two-photon excited fluorescence images provided highly resolved and thus topologically detailed information around the tumor regions where HerGa accumulates. Taken together, the results shown in this report suggest the feasibility of tumor detection and delineation by multimode optical imaging of HerGa, and fluorescent chemotherapy agents in general. Specifically, the multimode optical imaging can offer complementary and even synergetic information simultaneously in the tumor detection and delineation by HerGa, thus enhancing contrast.


Item Type:Book Section
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1117/12.877780DOIArticle
http://proceedings.spiedigitallibrary.org/proceeding.aspx?articleid=718972PublisherArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4582672/PubMed CentralArticle
Additional Information:© 2011 SPIE. Partial support from the US Navy Bureau of Medicine and Surgery is gratefully acknowledged. This work was partially supported by grants from the NIH (R21 CA116014, R01 CA102126, R01 CA129822, and R01 CA140995), the DoD (BC050662), the Susan G. Komen Breast Cancer foundation (BCTR0201194), and the Donna and Jesse Garber Award. Work at Caltech was supported by NIH DK019038 and the Arnold and Mabel Beckman Foundation. Work at the Technion was supported by The Herbert Irving Cancer and Atherosclerosis Research Fund.
Funders:
Funding AgencyGrant Number
US Navy Bureau of Medicine and SurgeryUNSPECIFIED
NIHR21 CA116014
NIHR01 CA102126
NIHR01 CA129822
NIHR01 CA140995
Department of DefenseBC050662
Susan G. Komen Breast Cancer FoundationBCTR0201194
Donna and Jesse Garber AwardUNSPECIFIED
NIHDK019038
Arnold and Mabel Beckman FoundationUNSPECIFIED
Herbert Irving Cancer and Atherosclerosis Research FundUNSPECIFIED
Subject Keywords:Multimode optical imaging, two-photon excitation, spectral imaging, fluorescence lifetime imaging microscopy (FLIM), tumor targeted corrole, tumor detection
PubMed Central ID:PMC4582672
Record Number:CaltechAUTHORS:20170111-103042462
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20170111-103042462
Official Citation:Jae Youn Hwang ; Zeev Gross ; Harry B. Gray ; Lali K. Medina-Kauwe ; Daniel L. Farkas; Multimode optical imaging for translational chemotherapy: in vivo tumor detection and delineation by targeted gallium corroles. Proc. SPIE 7902, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IX, 79020F (February 28, 2011); doi:10.1117/12.877780
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:73420
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:21 Jan 2017 01:54
Last Modified:21 Jan 2017 01:54

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