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Feedback localization of freely diffusing fluorescent particles near the optical shot-noise limit

Berglund, Andrew J. and McHale, Kevin and Mabuchi, Hideo (2007) Feedback localization of freely diffusing fluorescent particles near the optical shot-noise limit. Optics Letters, 32 (2). pp. 145-147. ISSN 0146-9592. https://resolver.caltech.edu/CaltechAUTHORS:BERol07

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Abstract

We report near-optimal tracking of freely diffusing fluorescent particles in a quasi-two-dimensional geometry via photon counting and real-time feedback. We present a quantitative statistical model of our feedback network and find excellent agreement with the experiment. We monitor the motion of a single fluorescent particle with a sensitivity of 15 nm/sqrt Hz while collecting fewer than 5000 fluorescence photons/s. Fluorescent microspheres (diffusion coefficient 1.3 μm^2/s) are tracked with a root-mean-square tracking error of 170 nm, within a factor of 2 of the theoretical limit set by photon counting shot noise.


Item Type:Article
Additional Information:© 2006 Optical Society of America. Received August 7, 2006; revised October 10, 2006; accepted October 12, 2006; posted October 18, 2006 (Doc. ID 73852); published December 23, 2006 K. McHale acknowledges support from an NDSEG fellowship. This work was supported by NSF grant CCF-0323542 and by the Institute for Collaborative Biotechnologies through grant DAAD19-03-D-0004 from the Army Research Office. Any opinions, findings, and conclusions or recommendations expressed in this material are those of the authors, and do not necessarily reflect the views of either funding agency.
Subject Keywords:Fluorescence microscopy; Scanning microscopy; Laser-induced fluorescence
Issue or Number:2
Record Number:CaltechAUTHORS:BERol07
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:BERol07
Alternative URL:http://www.opticsinfobase.org/abstract.cfm?URI=ol-32-2-145
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:7350
Collection:CaltechAUTHORS
Deposited By: Archive Administrator
Deposited On:02 Feb 2007
Last Modified:02 Oct 2019 23:41

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