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Amphiphilic Corroles Bind Tightly to Human Serum Albumin

Mahammed, Atif and Gray, Harry B. and Weaver, Jeremy J. and Sorasaenee, Karn and Gross, Zeev (2004) Amphiphilic Corroles Bind Tightly to Human Serum Albumin. Bioconjugate Chemistry, 15 (4). pp. 738-746. ISSN 1043-1802. https://resolver.caltech.edu/CaltechAUTHORS:20170125-093851899

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Abstract

Amphiphilic 2,17-bis-sulfonato-5,10,15(trispentafluorophenyl)corrole (2) and its Ga and Mn complexes (2-Ga and 2-Mn) form tightly bound noncovalent conjugates with human serum albumin (HSA). Protein-induced changes in the electronic absorption, emission, and circular dichroism spectra of these corroles, as well as results obtained from HPLC profiles of the conjugates and selective fluorescence quenching of the single HSA tryptophan, are interpreted in terms of multiple corrole:HSA binding sites. High-affinity binding sites, close to the unique tryptophan, are fully occupied at very low concentrations. At biologically relevant HSA concentrations (2−3 orders of magnitude larger than those employed in our studies), all corroles (2, 2-Ga, and 2-Mn) may be considered as fully conjugated.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1021/bc034179pDOIArticle
http://pubs.acs.org/doi/abs/10.1021/bc034179pPublisherArticle
ORCID:
AuthorORCID
Gray, Harry B.0000-0002-7937-7876
Sorasaenee, Karn0000-0002-3364-1918
Additional Information:© 2004 American Chemical Society. Received 30 September 2003; Published online 19 June 2004; Published in print 1 July 2004. Work at the Technion was supported by the STAR (Chicago) Foundation (Z.G.) and the Israel Science Foundation (Z.G.); work at Caltech was supported by JPL-NASA (K.S.) and the National Science Foundation (H.B.G., J.J.W.).
Funders:
Funding AgencyGrant Number
STAR FoundationUNSPECIFIED
Israel Science Foundation (ISF)UNSPECIFIED
NASA/JPLUNSPECIFIED
NSFUNSPECIFIED
Issue or Number:4
Record Number:CaltechAUTHORS:20170125-093851899
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20170125-093851899
Official Citation:Amphiphilic Corroles Bind Tightly to Human Serum Albumin Atif Mahammed,†, Harry B. Gray,*,‡, Jeremy J. Weaver,‡, Karn Sorasaenee,‡ and, and Zeev Gross*,† Bioconjugate Chemistry 2004 15 (4), 738-746 DOI: 10.1021/bc034179p
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:73706
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:25 Jan 2017 18:59
Last Modified:09 Mar 2020 13:19

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